Tipton D A, Howell K J, Dabbous M K
Department of Oral Biology, College of Dentistry, University of Tennesse, Memphis, USA.
J Periodontol. 1997 Jun;68(6):524-30. doi: 10.1902/jop.1997.68.6.524.
HEREDITARY GINGIVAL FIBROMATOSIS (HGF) is a fibrotic enlargement of the gingiva. HGF gingiva contains large amounts of interstitial collagen and other extracellular matrix (ECM) molecules. Increased proliferation and elevated production of the ECM molecules type I collagen and fibronectin (FN) could contribute to the clinical increased bulk of HGF gingiva. Fibroblast strains from HGF gingiva and normal human gingival fibroblast strains (GN) were used in this in vitro study. Fibroblast proliferation was determined by ELISA which measured the incorporation of 5-bromo-2'-deoxyuridine into DNA. The results showed that HGF fibroblast strains proliferated more rapidly than GN fibroblasts (68% to 488% increase, depending on the strains) (P < or = 0.01), the only exception being one HGF strain versus one normal strain. All HGF strains produced greater amounts of FN (measured by ELISA) than all of the normal fibroblast strains (23% to 49% increase, depending on the strain) (P < or = 0.04). Similarly, all HGF strains made significantly greater (P < or = 0.3) amounts of type I collagen (also measured by ELISA) than all of the normal strains (55% to 235% increase, depending on the strain). The results show that, in vitro, HGF fibroblasts display several phenotypic characteristics of activated fibroblasts: increased proliferative rates as well as increased production of FN and type I collagen, consistent with in vitro studies of fibroblasts derived from other types of fibrotic tissue. These results suggest that the increased proliferation of HGF fibroblasts and their increased production of extracellular matrix molecules such as collagen and FN may contribute to the clinical gingival enlargement characteristics of HGF.
遗传性牙龈纤维瘤病（HGF）是牙龈的纤维化肿大。HGF牙龈含有大量的间质胶原和其他细胞外基质（ECM）分子。I型胶原和纤连蛋白（FN）等ECM分子的增殖增加和产量升高可能导致HGF牙龈临床上的体积增大。本体外研究使用了来自HGF牙龈的成纤维细胞系和正常人牙龈成纤维细胞系（GN）。通过ELISA测定5-溴-2'-脱氧尿苷掺入DNA的情况来确定成纤维细胞增殖。结果显示，HGF成纤维细胞系比GN成纤维细胞增殖更快（增加68%至488%，取决于细胞系）（P≤0.01），唯一的例外是一个HGF细胞系与一个正常细胞系相比。所有HGF细胞系产生的FN量（通过ELISA测量）均高于所有正常成纤维细胞系（增加23%至49%，取决于细胞系）（P≤0.04）。同样，所有HGF细胞系产生的I型胶原量（也通过ELISA测量）均显著高于所有正常细胞系（增加55%至235%，取决于细胞系）（P≤0.03）。结果表明，在体外，HGF成纤维细胞表现出活化成纤维细胞的几种表型特征：增殖率增加以及FN和I型胶原产量增加，这与源自其他类型纤维化组织的成纤维细胞的体外研究一致。这些结果表明，HGF成纤维细胞增殖增加及其细胞外基质分子如胶原和FN产量增加可能导致HGF的临床牙龈肿大特征。
