cerexin A的氨基酸序列（芽孢杆菌属抗生素的研究。VII.）

The amino acid sequence of cerexin A (studies on antibiotics from the genus Bacillus. VII.

作者信息

Shoji J, Kato T

出版信息

J Antibiot (Tokyo). 1975 Oct;28(10):764-9. doi: 10.7164/antibiotics.28.764.

DOI:10.7164/antibiotics.28.764

Abstract

N-Bromosuccinimide cleavage reaction on cerexin A liberated allo-isoleucine. Treatment with conc. hydrochloric acid cleaved the antibiotic into two peptide fragments selectively at gamma-hydroxylysine residue. Deacylation with an enzyme preparation from Pseudomonas sp. afforded deacyl cerexin A. The amino acid sequences of these peptide fragments were examined by Edman degradation. From all the results, the entire amino acid sequence of cerexin A was deduced.

摘要

N-溴代琥珀酰亚胺对cerexin A的裂解反应释放出别异亮氨酸。用浓盐酸处理可使该抗生素在γ-羟基赖氨酸残基处选择性地裂解为两个肽片段。用假单胞菌属的一种酶制剂进行脱酰基反应得到脱酰基cerexin A。通过埃德曼降解法检测了这些肽片段的氨基酸序列。根据所有这些结果，推导得出了cerexin A的完整氨基酸序列。