Xie M, He Y X, Qiu L S, Chen W
Institute of Parasitic Disease, Chinese Academy of Preventive Medicine, Shanghai.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1993;11(1):6-8.
The nonradioactive labelled probe pSM 889 was hybridized to Southern blots of genomic DNA of five isolates of Schistosoma japonicum from Anhui, Hubei, Guangxi, Sichuan and Yunnan in the mainland of China. The major bands of hybridization of DNA digested by restriction endonucleases EcoRI and BamHI are same among the five isolates, but the minor bands of hybridization of DNA digested by EcoRI show some differences between 4.4-9.6 kb and between 2.3-4.4 kb among the five isolates. The result shows that the five isolates are of some genetic variation. It provides, at molecular level, evidence of the existence of different strains of S. japonicum in the mainland of China.
将非放射性标记探针pSM 889与中国大陆安徽、湖北、广西、四川和云南的五株日本血吸虫基因组DNA的Southern印迹进行杂交。经限制性内切酶EcoRI和BamHI消化的DNA杂交主要条带在这五株分离株中相同，但经EcoRI消化的DNA杂交次要条带在4.4 - 9.6 kb以及2.3 - 4.4 kb之间的五株分离株中存在一些差异。结果表明这五株分离株存在一定的遗传变异。它在分子水平上为中国大陆存在不同株日本血吸虫提供了证据。
