He Y X, Hu Y Q, Yu Q F, Ni C H, Xue H C, Qiu L S, Xie M
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.
Southeast Asian J Trop Med Public Health. 1994 Jun;25(2):232-42.
The present paper deals with studies on the characteristics of Schistosoma japonicum isolated from five localities in the mainland of China. The following items were observed and compared including morphometric data, susceptibility of six mammalian hosts, prepatent period, compatibility between larvae and snail hosts, size of hepatic granuloma produced by eggs, immunoreactions in experimental animals, sensitivity to praziquantel, SDS-PAGE protein pattern and its antigenicity analysis, DNA hybridization and genetic variation and differentiation by analysis with multilocus enzyme electrophoresis. By means of these multidisciplinary methods, from morphological to molecular level, the following conclusions may be drawn from our results. The evidence indicates firstly that S. japonicum in the mainland of China comprises a strain complex with several components of geographically distributed strains. At least four distinct strains exist, ie Yunnan, Guangxi, Sichuan and Anhui-Hubei. Characteristics of each strain are distinct and the results of these studies lead to discussion on the problem of the intraspecific and interstrain differentiation of S. japonicum in the mainland of China.
本文研究了从中国大陆五个地区分离出的日本血吸虫的特性。观察并比较了以下各项，包括形态测量数据、六种哺乳动物宿主的易感性、潜伏期、幼虫与螺宿主的相容性、虫卵引起的肝肉芽肿大小、实验动物的免疫反应、对吡喹酮的敏感性、SDS - PAGE蛋白图谱及其抗原性分析、DNA杂交以及通过多位点酶电泳分析的遗传变异和分化。通过这些从形态学到分子水平的多学科方法，我们的研究结果可得出以下结论。证据首先表明，中国大陆的日本血吸虫构成一个包含多个地理分布菌株成分的菌株复合体。至少存在四个不同的菌株，即云南株、广西株、四川株和安徽 - 湖北株。每个菌株的特性都不同，这些研究结果引发了对中国大陆日本血吸虫种内和株间分化问题的讨论。
