通过脉冲场凝胶电泳测定普氏立克次体和斑疹伤寒立克次体的基因组大小及限制性图谱多态性

Determination of genome size and restriction pattern polymorphism of Rickettsia prowazekii and Rickettsia typhi by pulsed field gel electrophoresis.

作者信息

Eremeeva M E, Roux V, Raoult D

机构信息

Unité des Rickettsies, Faculté de Médecine, Marseille, France.

出版信息

FEMS Microbiol Lett. 1993 Aug 15;112(1):105-12. doi: 10.1111/j.1574-6968.1993.tb06431.x.

DOI:10.1111/j.1574-6968.1993.tb06431.x

PMID:8104842

Abstract

Pulsed field gel electrophoresis (PFGE) of SmaI, MluI and SalI digested DNA was used to estimate genome size and perform restriction fragment length polymorphism analysis for Rickettsia prowazekii and Rickettsia typhi. We concluded that the genome of R. prowazekii and R. typhi consisted of a single chromosomal DNA. The total length of DNA of R. prowazekii was 1,106 +/- 54 kb and of R. typhi was 1,133 +/- 44 kb. It was possible to differentiate two strains of R. prowazekii, Breinl and EVir, by PFGE analysis after SalI digestion. Restriction fragment length polymorphism analysis did not reveal intraspecies differences between three human isolates and one Xenopsilla cheopis isolate of R. typhi.

摘要

使用经SmaI、MluI和SalI酶切的DNA进行脉冲场凝胶电泳（PFGE），以估计普氏立克次体和斑疹伤寒立克次体的基因组大小并进行限制性片段长度多态性分析。我们得出结论，普氏立克次体和斑疹伤寒立克次体的基因组由单一染色体DNA组成。普氏立克次体DNA的总长度为1106±54 kb，斑疹伤寒立克次体DNA的总长度为1133±44 kb。经SalI酶切后，通过PFGE分析可以区分普氏立克次体的两个菌株，即布雷尼尔菌株和EVir菌株。限制性片段长度多态性分析未揭示斑疹伤寒立克次体的三个人类分离株和一个印鼠客蚤分离株之间的种内差异。