斑疹伤寒群立克次体DNA的限制性片段长度多态性

Restriction fragment length polymorphism of the DNA of typhus group rickettsiae.

作者信息

Artemiev M I, Ignatovich V F, Rydkina E B, Lichoded L J, Balayeva N M, Demkin V V

机构信息

N. F. Gamaleya Research Institute of Epidemiology and Microbiology, U.S.S.R. Academy of Medical Sciences, Moscow.

出版信息

Acta Virol. 1991 Nov;35(6):526-30.

PMID:1687635

Abstract

The DNA of 10 strains of Rickettsia prowazekii, 5 strains of Rickettsia typhi and 1 strain of Rickettsia canada was investigated by restriction fragment length polymorphism analysis. Interspecies differences were characterized by a great number of noncomigrating bands. Using the endonuclease HindIII and PstI fragments comigration as a quantitative criterion, genetic similarity coefficient was calculated for the pair Rickettsia prowazekii/Rickettsia typhi-32.0%, for Rickettsia prowazekii/Rickettsia canada-22.7%, and for Rickettsia typhi/Rickettsia canada-23.5%. Intraspecies differences expressed are very subtle and concern 1-2 noncomigrating fragments. The investigated strains of Rickettsia prowazekii and Rickettsia typhi can be divided into 2 groups without any correlation to the source and period of isolation, or to strain passage history.

摘要

通过限制性片段长度多态性分析研究了10株普氏立克次体、5株斑疹伤寒立克次体和1株加拿大立克次体的DNA。种间差异表现为大量不共迁移条带。以核酸内切酶HindIII和PstI片段共迁移作为定量标准，计算了普氏立克次体/斑疹伤寒立克次体对的遗传相似系数为32.0%，普氏立克次体/加拿大立克次体对为22.7%，斑疹伤寒立克次体/加拿大立克次体对为23.5%。种内差异非常细微，涉及1 - 2个不共迁移片段。所研究的普氏立克次体和斑疹伤寒立克次体菌株可分为两组，与分离来源和时期或菌株传代历史无关。