纯化的和细胞结合的CD26的抗体结合谱。BT5/9和TA5.9在CD26簇中的指定。

Antibody binding profile of purified and cell-bound CD26. Designation of BT5/9 and TA5.9 to the CD26 cluster.

作者信息

de Meester I, Scharpé S, Vanham G, Bosmans E, Heyligen H, Vanhoof G, Corte G

机构信息

Department of Medical Biochemistry, University of Antwerp, Wilrijk, Belgium.

出版信息

Immunobiology. 1993 Jun;188(1-2):145-58. doi: 10.1016/S0171-2985(11)80494-8.

DOI:10.1016/S0171-2985(11)80494-8

PMID:8104877

Abstract

The CD26 activation antigen (Ag) which is expressed on a subpopulation of human T cells has been characterized as dipeptidyl peptidase IV (DPP IV, EC 3.4.14.5). In this paper, we describe the antibody binding profile of CD26/DPP IV, purified from human peripheral blood lymphocytes. The purified molecule binds to the anti-Ta1, anti-1F7 and anti-134-2C2 monoclonal antibodies (mAb), reported to react with cell-bound CD26 Ag. Among unclustered mAb recognizing T cell antigens, two, anti-BT5/9 and anti-TA5.9 were found to react with purified and cell-bound CD26 Ag. The classification of the BT5/9 Ag, the functional properties of the BT5/9+ T cell subset, as well as the in vivo effect of anti-BT5/9 mAb administration, are re-interpreted in the light of its specificity. Applying the anti-TA5.9 mAb in three color FACS analyses, we demonstrated that CD26+bright cells co-express CD45RO but not HLA-DR and CD38.

摘要

在人类T细胞亚群上表达的CD26活化抗原（Ag）已被鉴定为二肽基肽酶IV（DPP IV，EC 3.4.14.5）。在本文中，我们描述了从人外周血淋巴细胞中纯化的CD26/DPP IV的抗体结合谱。纯化的分子与据报道可与细胞结合的CD26 Ag反应的抗Ta1、抗1F7和抗134-2C2单克隆抗体（mAb）结合。在识别T细胞抗原的未聚类mAb中，发现两种，即抗BT5/9和抗TA5.9与纯化的和细胞结合的CD26 Ag反应。根据其特异性，对BT5/9 Ag的分类、BT5/9+ T细胞亚群的功能特性以及抗BT5/9 mAb给药的体内效应进行了重新解释。在三色荧光激活细胞分选分析中应用抗TA5.9 mAb，我们证明CD26+亮细胞共表达CD45RO，但不表达HLA-DR和CD38。