Mattern T, Ansorge S, Flad H D, Ulmer A J
Department of Immunology and Cell Biology, Forschungsinstitut Borstel, Germany.
Immunobiology. 1993 Jun;188(1-2):36-50. doi: 10.1016/S0171-2985(11)80485-7.
Three different anti-CD26 monoclonal antibodies (mAbs) are described, which specifically inhibited proliferation of human T lymphocytes after stimulation with PHA, tetanus toxoid or soluble anti-CD3 mAb. Anti-CD26 mAbs induced in T cells a dose-dependent shift of the maximum of DNA synthesis, which was due to a transitory arrest of cells in the cell cycle. This cell cycle arrest was found to occur in the late phase of G1 since the expression of the T cell activation markers CD25-, CD71-, and HLA-DR-positive cells was the same in anti-CD26 mAb-containing and control cultures. Propidium iodide staining further confirmed the assumption that the arrest occurs in the first round of the cell cycle before S phase cells were detectable. Because the cells were arrested before consuming IL-2 in the S phase, we observed in accumulation of IL-2 in anti-CD26 mAb-containing cultures, whereas IFN-gamma production by PHA-activated T lymphocytes was reduced. These data indicate that CD26 is involved in the processes of T cell activation and proliferation.
本文描述了三种不同的抗CD26单克隆抗体（mAb），它们在人T淋巴细胞经PHA、破伤风类毒素或可溶性抗CD3 mAb刺激后，能特异性抑制其增殖。抗CD26 mAb在T细胞中诱导DNA合成最大值出现剂量依赖性偏移，这是由于细胞在细胞周期中短暂停滞所致。发现这种细胞周期停滞发生在G1期后期，因为在含抗CD26 mAb的培养物和对照培养物中，T细胞活化标志物CD25、CD71和HLA-DR阳性细胞的表达相同。碘化丙啶染色进一步证实了停滞发生在细胞周期第一轮且在可检测到S期细胞之前的假设。由于细胞在S期消耗IL-2之前就停滞了，我们观察到含抗CD26 mAb的培养物中IL-2积累，而PHA激活的T淋巴细胞产生的IFN-γ减少。这些数据表明CD26参与了T细胞活化和增殖过程。
