Hesketh J, Dobbelaere D, Griffin J F, Buchan G
Department of Microbiology, University of Otago, Dunedin, New Zealand.
Immunology. 1993 Jul;79(3):465-70.
The expression of interleukin-2 receptors (IL-2R) and proliferating cell nuclear antigens (PCNA) were compared for their usefulness as markers of lymphocyte activation. Heterologous polyclonal (anti-bovine IL-2R) and monoclonal (anti-human PCNA) antibodies were used to detect the expression of these molecules on activated deer lymphocytes. Both molecules were co-expressed on blast cells which had been activated with mitogen [concanavalin A (Con A)]. There was detectable up-regulation of IL-2R expression in response to antigen [Mycobacterium bovis-derived purified protein derivative (PPD)] stimulation while PCNA expression mimicked lymphocyte transformation (LT) reactivity. PCNA expression was found to more accurately reflect both antigen- and mitogen-activated lymphocyte activation, as estimated by LT activity. The expression of PCNA was used to identify antigen reactive cells from animals exposed to M. bovis. A very low percentage (1.1 +/- 0.4%) of peripheral blood lymphocytes from non-infected animals could be stimulated to express PCNA by in vitro culture with antigen (PPD). Within the infected group both diseased and healthy, 'in-contact', animals expressed significantly higher levels of PCNA upon antigen stimulation.
比较白细胞介素-2受体（IL-2R）和增殖细胞核抗原（PCNA）作为淋巴细胞活化标志物的实用性。使用异源多克隆抗体（抗牛IL-2R）和单克隆抗体（抗人PCNA）检测这些分子在活化的鹿淋巴细胞上的表达。这两种分子在经丝裂原[刀豆球蛋白A（Con A）]活化的母细胞上共表达。在抗原[牛分枝杆菌衍生的纯化蛋白衍生物（PPD）]刺激下，可检测到IL-2R表达上调，而PCNA表达模拟淋巴细胞转化（LT）反应性。通过LT活性估计，发现PCNA表达能更准确地反映抗原和丝裂原激活的淋巴细胞活化情况。PCNA表达用于识别暴露于牛分枝杆菌的动物体内的抗原反应性细胞。通过与抗原（PPD）体外培养，未感染动物外周血淋巴细胞中只有极低比例（1.1±0.4%）能被刺激表达PCNA。在感染组中，患病和健康的“接触感染”动物在抗原刺激后PCNA表达水平均显著升高。
