在辛德毕斯病毒感染的BHK细胞中多聚核糖体RNA的鉴定
Identification of polysomal RNA in BHK cells infected by sindbis virus.
作者信息
Mowshowitz D
出版信息
J Virol. 1973 Apr;11(4):535-43. doi: 10.1128/JVI.11.4.535-543.1973.
Abstract
Polysomes were prepared from Sindbis virus-infected BHK cells. The major species of RNA in these polysomes was identified as 26S RNA (interjacent RNA) by (i) disrupting the polysomes with EDTA; (ii) treating the infected cells with puromycin; and (iii) isolating polysomes from cells infected with a temperature-sensitive mutant that does not form nucleocapsids. Small amounts of 42S RNA and 33S RNA were also found in polysomes.
摘要
从辛德毕斯病毒感染的BHK细胞中制备多核糖体。通过以下方法将这些多核糖体中的主要RNA种类鉴定为26S RNA（居间RNA）：（i）用乙二胺四乙酸（EDTA）破坏多核糖体；（ii）用嘌呤霉素处理感染的细胞；（iii）从感染了不形成核衣壳的温度敏感突变体的细胞中分离多核糖体。在多核糖体中还发现了少量的42S RNA和33S RNA。
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