Saintigny G, Bonnard M, Damour O, Collombel C
Laboratoire des Substituts Cutanés, Hôpital Edouard Herriot, Lyon, France.
Acta Derm Venereol. 1993 Jun;73(3):175-80. doi: 10.2340/0001555573175180.
Reconstruction of epidermis on a fibroblast containing chitosan cross-linked collagen-GAG lattice at the air-liquid interface gives rise to a multilayered stratified epithelium, covered with a compact stratum corneum. Immunohistological studies reveal that the markers of epidermal differentiation are essentially distributed as in normal human skin and that the major proteins of the dermal-epidermal junction are present. Reconstruction of epidermis under identical culture conditions on a dermal equivalent that does not contain fibroblasts gives rise to an epithelium consisting of disorganized cell layers where the markers of differentiation are either displaced or not at all expressed, as in the case with filaggrin. In contrast, expression of the major constituents of the dermal-epidermal junction by the keratinocytes is completely independent of the presence of fibroblasts, even though it seems that the presence of fibroblasts is essential for the organization of the basement membrane proteins, creating a tight junction between dermal equivalent and the reconstructed epidermis.
在气液界面上，于含有壳聚糖交联胶原 - 糖胺聚糖晶格的成纤维细胞上重建表皮，可形成多层分层上皮，其表面覆盖有致密的角质层。免疫组织学研究表明，表皮分化标志物的分布与正常人类皮肤基本相同，且存在真皮 - 表皮连接的主要蛋白质。在相同培养条件下，于不含成纤维细胞的真皮替代物上重建表皮，会形成由无序细胞层组成的上皮，其中分化标志物要么移位，要么根本不表达，如丝聚蛋白的情况。相比之下，角质形成细胞对真皮 - 表皮连接主要成分的表达完全独立于成纤维细胞的存在，尽管成纤维细胞的存在似乎对基底膜蛋白的组织至关重要，能在真皮替代物与重建表皮之间形成紧密连接。
