Egelrud T, Régnier M, Sondell B, Shroot B, Schmidt R
L'Oréal Centre International, Valbonne, France.
Acta Derm Venereol. 1993 Jun;73(3):181-4. doi: 10.2340/0001555573181184.
The aim of the present study was to investigate the presence of stratum corneum chymotryptic enzyme (SCCE) in human epidermis reconstructed in vitro on dead de-epidermized dermis and the effect of retinoic acid (RA) on its expression. SCCE is a recently discovered serine proteinase which has been purified from human stratum corneum, and evidence has been presented that it may play a role in stratum corneum turnover, especially in desquamation. The SCCE-specific monoclonal antibody TE-9B showed positive immunofluorescence staining of high suprabasal keratinocytes, mainly in the stratum granulosum, in normal non-palmo-plantar human epidermis as well as in reconstructed epidermis in the absence of RA. This staining was also seen in reconstructed epidermis cultured in the presence of 10(-8) M RA, a concentration at which the reconstructed epidermis still formed an orthokeratotic stratum corneum. At 10(-7) M RA, however, not only the formation of the stratum corneum but also SCCE expression was suppressed. These results support the hypothesis that SCCE expression is related to and may be part of the epidermis-specific differentiation program, where the enzyme may be involved in the homeostasis of the stratum corneum, possibly by degrading the intercorneocyte cohesive structures.
本研究的目的是调查在脱细胞真皮上体外重建的人表皮中是否存在角质层糜蛋白酶（SCCE）以及视黄酸（RA）对其表达的影响。SCCE是一种最近从人角质层中纯化出来的丝氨酸蛋白酶，有证据表明它可能在角质层周转中起作用，尤其是在脱屑过程中。SCCE特异性单克隆抗体TE-9B在正常非掌跖部人表皮以及无RA的重建表皮中，对高基底层上方的角质形成细胞呈现阳性免疫荧光染色，主要在颗粒层。在10^(-8) M RA存在的情况下培养的重建表皮中也可见这种染色，在该浓度下重建表皮仍形成正角化的角质层。然而，在10^(-7) M RA时，不仅角质层的形成受到抑制，SCCE的表达也受到抑制。这些结果支持了这样的假设，即SCCE的表达与表皮特异性分化程序相关，并且可能是其一部分，在该程序中该酶可能通过降解角质形成细胞间的黏附结构参与角质层的稳态维持。
