在微载体上的细胞培养中大规模生产传染性牛鼻气管炎病毒。
Large-scale production of infectious bovine rhinotracheitis virus in cell culture on microcarriers.
作者信息
Lesko J, Veber P, Hrda M, Feketeová M
机构信息
Institute of Virology, Slovak Academy of Sciences, Bratislava.
出版信息
Acta Virol. 1993 Feb;37(1):73-8.
PMID:8105653
Abstract
Large amounts of infectious bovine rhinotracheitis (IBR) virus was produced in Madin-Darby bovine kidney (MDBK) cells in suspension culture on the microcarriers Gelaspher M. Virus yields (TCID50 per ml) were approximately 10-times higher as compared to classical stationary cell culture.
摘要
在微载体Gelaspher M上进行悬浮培养的马-达二氏牛肾（MDBK）细胞中产生了大量传染性牛鼻气管炎（IBR）病毒。与传统的固定细胞培养相比，病毒产量（每毫升的半数组织培养感染剂量）大约高出10倍。
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