Microbiology and Epidemiology Group, Faculty of Veterinary Medicine and Animal Science, National University of Colombia, Bogotá 11001, Colombia.
Virol Sin. 2013 Feb;28(1):36-42. doi: 10.1007/s12250-013-3283-z. Epub 2013 Feb 6.
Bovine Herpesvirus-1 (BoHV-1) is a DNA virus belonging to the family Herpesviridae, subfamily Alfaherpesvirinae; it is a worldwide pathogen, causing serious economic losses in livestock. In Colombia there have been multiple isolates of BoHV-1 that have been subjected to molecular characterization, classifying most of the country isolates as BoHV-1.1. In the present study we developed and evaluated an ethyleneimine binary inactivated isolate from the native BoHV-1 strain (Córdoba-2) in a rabbit model of vaccination and infection. The vaccine was evaluated in two phases, one of immunogenicity with vaccination and a booster after 21 days, and an evaluation phase of protection against challenge with a highly virulent reference strain. The results demonstrate optimum serum-conversion, with protective neutralizing antibody titers 28 days post vaccination and optimal protection against challenge with the reference strain with decreased clinical signs of infection, protection against the onset of fever and decrease of virus excretion post challenge. In conclusion, our results show the enormous potential that an immunogenic inactivated vaccine has produced from the native BoHV-1.1 strain, which produces a high antigen mass to the vaccine to induce optimal immunity and protection, and it is a strong candidate for evaluation and possible future use in different cattle populations.
牛疱疹病毒 1 型（BoHV-1）是一种 DNA 病毒，属于疱疹病毒科，α疱疹亚科；它是一种全球性病原体，给家畜造成严重的经济损失。在哥伦比亚，已经有多种 BoHV-1 分离株进行了分子特征分析，其中大多数国家的分离株被归类为 BoHV-1.1。在本研究中，我们使用本地 BoHV-1 株（Córdoba-2）开发并评估了一种乙烯亚胺二联灭活株，在兔接种和感染模型中进行了评估。该疫苗在两个阶段进行了评估，一个是免疫原性阶段，接种后 21 天进行加强免疫，另一个是保护效力评估阶段，用高毒力的参考株进行攻毒挑战。结果表明，该疫苗具有最佳的血清转化率，接种后 28 天产生保护性中和抗体滴度，对参考株的攻毒具有最佳的保护作用，可降低感染的临床症状、发热的发生和攻毒后病毒的排出。总之，我们的结果表明，一种来自本地 BoHV-1.1 株的免疫原性灭活疫苗具有巨大的潜力，它产生大量的抗原，可诱导最佳的免疫和保护效果，是评估和未来在不同牛群中使用的强有力候选疫苗。
