通过增殖细胞核抗原/细胞周期蛋白的表达及有丝分裂率比较Spitz痣、黑素细胞痣和恶性黑色素瘤中的增殖活性。

Proliferative activities in Spitz nevus compared with melanocytic nevus and malignant melanoma using expression of PCNA/cyclin and mitotic rate.

作者信息

Tu P, Miyauchi S, Miki Y

机构信息

Department of Dermatology, University of Ehime School of Medicine, Japan.

出版信息

Am J Dermatopathol. 1993 Aug;15(4):311-4. doi: 10.1097/00000372-199308000-00003.

DOI:10.1097/00000372-199308000-00003

PMID:8105711

Abstract

Proliferative activity in Spitz nevus (SN) was determined using proliferating cell nuclear antigen (PCNA) immunostaining and by assessing the mitotic rate. It was compared with that in compound melanocytic nevus (MN) and in malignant melanoma (MM). The PCNA index (number of positive cells/1,000 tumor cells) in SN was 72.8 +/- 45.5% (mean +/- SD), which was statistically significantly higher than that in MN (7.2 +/- 2.7%) and lower than that in MM (248.5 +/- 110%). The PCNA-positive cells in SN and MM were found both in dermal and junctional nests, while those in MN were found almost exclusively in the dermal nests. The mitotic rate in SN was 1.4 +/- 0.96%, while it was nothing in MN and 5.4 +/- 4.2% in MM. There was a statistically significant correlation between the PCNA index and the mitotic rate at r = 0.8946, p < 0.001. The PCNA index, however, appeared to show the proliferative activity of SN more clearly than did the mitotic rate.

摘要

通过增殖细胞核抗原（PCNA）免疫染色和评估有丝分裂率来确定Spitz痣（SN）中的增殖活性。并将其与复合性黑素细胞痣（MN）和恶性黑色素瘤（MM）中的增殖活性进行比较。SN中的PCNA指数（阳性细胞数/1000个肿瘤细胞）为72.8±45.5%（平均值±标准差），在统计学上显著高于MN中的PCNA指数（7.2±2.7%），但低于MM中的PCNA指数（248.5±110%）。SN和MM中的PCNA阳性细胞在真皮巢和交界巢中均有发现，而MN中的PCNA阳性细胞几乎仅在真皮巢中发现。SN的有丝分裂率为1.4±0.96%，而MN中无有丝分裂，MM中的有丝分裂率为5.4±4.2%。PCNA指数与有丝分裂率之间存在统计学上的显著相关性，r = 0.8946，p < 0.001。然而，PCNA指数似乎比有丝分裂率更能清楚地显示SN的增殖活性。