Yeung R S, Penninger J, Mak T W
University of Toronto, Ontario Cancer Institute, Canada.
Curr Opin Immunol. 1993 Aug;5(4):585-94. doi: 10.1016/0952-7915(93)90042-q.
Mouse strains with defined genetic defects engineered by the method of targeted gene disruption and homologous recombination have furthered our understanding of immune functions at the single gene level. More importantly, these mutant 'gene knockout' mice are powerful in vivo tools to dissect the complex mechanisms of lymphocyte development and function, complementing our broadening knowledge of congenital and acquired human immunodeficiencies.
通过靶向基因破坏和同源重组方法构建的具有特定遗传缺陷的小鼠品系，进一步加深了我们在单基因水平上对免疫功能的理解。更重要的是，这些突变的“基因敲除”小鼠是剖析淋巴细胞发育和功能复杂机制的强大体内工具，补充了我们对先天性和获得性人类免疫缺陷不断拓宽的认识。
