Imai K, Aimoto T, Sato M, Kimura R
Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Biol Pharm Bull. 1993 Aug;16(8):759-61. doi: 10.1248/bpb.16.759.
The effect of protoporphyrin (PP) administration on the activities of enzymes related to and/or involved in lipid peroxidation and on the content of reduced glutathione (GSH) was investigated in rat liver. PP, at an intravenous dose of 20 mg/kg, increased GSH content, caused a weak suppression of NADPH-cytochrome c reductase activity and a slight increase of gamma-glutamyl transpeptidase activity 24 h after dosing, but had no effect on the activities of other enzymes such as xanthine oxidase, superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione S-transferase, gamma-glutamylcysteine synthetase or glutathione synthetase. Treatment of rats with diethyl maleate following PP injection resulted in the disappearance of antioxidative action of PP. Furthermore, sinusoidal, but not canalicular, efflux of hepatic GSH was decreased by the PP treatment. The increase of liver GSH content by PP treatment due to the decrease of sinusoidal efflux of GSH from the liver, thus would be involved in the exertion of antioxidative action of PP.
在大鼠肝脏中研究了给予原卟啉（PP）对与脂质过氧化相关和/或参与脂质过氧化的酶活性以及还原型谷胱甘肽（GSH）含量的影响。静脉注射剂量为20mg/kg的PP，给药24小时后可增加GSH含量，对NADPH-细胞色素c还原酶活性有微弱抑制作用，γ-谷氨酰转肽酶活性略有增加，但对其他酶如黄嘌呤氧化酶、超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽S-转移酶、γ-谷氨酰半胱氨酸合成酶或谷胱甘肽合成酶的活性没有影响。PP注射后用马来酸二乙酯处理大鼠导致PP的抗氧化作用消失。此外，PP处理可降低肝GSH的窦状隙（而非胆小管）流出。因此，由于肝脏GSH窦状隙流出减少，PP处理导致肝脏GSH含量增加，这可能参与了PP抗氧化作用的发挥。
