Imai K, Aimoto T, Sato M, Kimura R
Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
J Pharmacobiodyn. 1991 Jan;14(1):20-4. doi: 10.1248/bpb1978.14.20.
Effect of intravenous administration of protoporphyrin IX (PP) on lipid peroxidation was studied in rats. PP and/or PP-derived porphyrins were found to be mainly distributed in livers, spleen and lungs. Dose-dependent decreases in the Fe2+ and L-ascorbic acid-stimulated lipid peroxidation in homogenates of livers and dose-dependent increases in porphyrin concentration in livers were observed after the PP injection. In the experiments with a 20 mg/kg dose of PP, the peroxidation level in the liver homogenates reached its minimum level during the period of 3 to 24 h accompanying the high porphyrin concentration in livers after the administration. After 96 h, a relatively high porphyrin concentration was still retained, but decreases in the peroxidation levels had ceased. PP administration caused a dose-dependent decrease in the endogenous lipid peroxides in livers within 0.5 h and the low levels were maintained throughout the course of the 168-h study. These results clearly show that the administered PP is distributed in the liver and inhibits the lipid peroxidation in vivo.
研究了静脉注射原卟啉IX（PP）对大鼠脂质过氧化的影响。发现PP和/或PP衍生的卟啉主要分布在肝脏、脾脏和肺中。注射PP后，观察到肝脏匀浆中Fe2+和L-抗坏血酸刺激的脂质过氧化呈剂量依赖性降低，且肝脏中卟啉浓度呈剂量依赖性增加。在20mg/kg剂量的PP实验中，给药后3至24小时内，肝脏匀浆中的过氧化水平达到最低水平，同时肝脏中卟啉浓度较高。96小时后，卟啉浓度仍相对较高，但过氧化水平的降低已停止。PP给药在0.5小时内导致肝脏中内源性脂质过氧化物呈剂量依赖性降低，且在168小时的研究过程中一直维持在低水平。这些结果清楚地表明，给药的PP分布在肝脏中并抑制体内脂质过氧化。
