Allen R L, Lonsdale D M
Cambridge Laboratory, John Innes Centre for Plant Science Research, Norwich, Norfolk, UK.
Plant J. 1993 Feb;3(2):261-71. doi: 10.1111/j.1365-313x.1993.tb00177.x.
A gene exhibiting homology to the polygalacturonases of several species, including tomato and Oenothera, has been shown by RNA dot-blot analysis and in situ hybridization experiments to be expressed post-first microspore mitosis in maize. A 2.87 kbp section of the promoter fused to E. coli beta-glucuronidase (uidA) coding sequence conferred the correct spatial and temporal expression in transgenic tobacco plants. However, low levels of expression were detected in other tissues, and in particular in the tissues surrounding the vascular branch points of leaf nodes. The maize polygalacturonase gene is one member of a highly conserved gene family. The lack of detectable expression in sporophytic tissues and the isolation of a number of related cDNAs from maize suggests that all expressed members of this family show the same spatial and temporal regulation.
一个与包括番茄和月见草在内的多个物种的多聚半乳糖醛酸酶具有同源性的基因，已通过RNA斑点杂交分析和原位杂交实验表明，在玉米中首次小孢子有丝分裂后表达。一段2.87kbp的启动子片段与大肠杆菌β-葡萄糖醛酸酶（uidA）编码序列融合，在转基因烟草植株中赋予了正确的时空表达。然而，在其他组织中检测到低水平的表达，特别是在叶节维管束分支点周围的组织中。玉米多聚半乳糖醛酸酶基因是一个高度保守的基因家族的成员之一。在孢子体组织中缺乏可检测到的表达，以及从玉米中分离出一些相关的cDNA，表明该家族所有表达的成员都表现出相同的时空调控。
