John M E, Petersen M W
Agracetus, Inc., University Green Middleton, WI 53562.
Plant Mol Biol. 1994 Dec;26(6):1989-93. doi: 10.1007/BF00019509.
A gene (G9) expressed during late microsporogenesis in cotton (Gossypium hirsutum L.) was isolated. Sequence analysis of the cDNA (1.3 kb) as well as the gene (2.6 kb) revealed an open reading frame of 1233 bases encoding a protein of 43.9 kDa. The coding region of the gene is interrupted by three introns. Northern analysis of the RNA from developing anthers showed that the transcripts appear 12 days before anthesis and that the maximal concentration of RNA occurs in pollen on the day of anthesis. This pattern of gene expression suggests functions in post-anthesis events. Sequence comparisons with other known plant genes indicated that G9 is homologous to polygalacturonases. The G9 promoter conferred tissue and temporal specificity of beta-glucuronidase (GUS) expression in transgenic tobacco plants. Thus, the G9 promoter can be used to drive gene expression in homologous as well as heterologous plants in a tissue-specific manner.
从棉花（陆地棉）小孢子发生后期表达的一个基因（G9）被分离出来。对该cDNA（1.3 kb）和基因（2.6 kb）的序列分析揭示了一个1233个碱基的开放阅读框，编码一个43.9 kDa的蛋白质。该基因的编码区被三个内含子打断。对发育中的花药RNA进行的Northern分析表明，转录本在开花前12天出现，并且RNA的最大浓度出现在开花当天的花粉中。这种基因表达模式表明其在开花后事件中发挥作用。与其他已知植物基因的序列比较表明，G9与多聚半乳糖醛酸酶同源。G9启动子在转基因烟草植株中赋予了β-葡萄糖醛酸酶（GUS）表达的组织和时间特异性。因此，G9启动子可用于以组织特异性方式驱动同源及异源植物中的基因表达。
