van Eijk H M, Rooyakkers D R, Deutz N E
Department of Surgery, University of Limburg, Maastricht, Netherlands.
J Chromatogr. 1993 Oct 22;620(1):143-8. doi: 10.1016/0378-4347(93)80062-9.
A rapid fully automated method for the determination of amino acids is described based on high-performance liquid chromatography and pre-column o-phthaldialdehyde derivatization. Using a 150 mm x 4.6 mm I.D. HPLC column filled with a recently introduced 2-3 microns Spherisorb ODS II packing material, 30 physiological amino acids could be determined within 28 min (injection to injection), while 95 samples could be processed unattended within 45 h. For most amino acids, the coefficient of variation (C.V.) for the peak areas measured was below 3%, both in aqueous standards and in plasma. Providing a pre-column change every 200-300 runs, the separation remained unaltered for about 1500-2000 runs on a single column.
本文描述了一种基于高效液相色谱和柱前邻苯二甲醛衍生化的快速全自动氨基酸测定方法。使用内径为4.6 mm、长度为150 mm的HPLC柱，填充最近推出的2 - 3微米的Spherisorb ODS II填充材料，可在28分钟内（进样到进样）测定30种生理性氨基酸，同时可在45小时内无人值守处理95个样品。对于大多数氨基酸，无论是在水标准品还是血浆中，所测峰面积的变异系数（C.V.）均低于3%。每运行200 - 300次更换一次柱前部分，在一根柱子上大约可进行1500 - 2000次运行，分离效果保持不变。
