Ault S J, Leventhal A G
Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City 84132.
J Comp Neurol. 1994 Jan 1;339(1):106-16. doi: 10.1002/cne.903390110.
Previous investigators have documented the postnatal development of alpha and beta type ganglion cells in cat retinae (Ramoa et al. [1987] Science 237:522-525; Ramoa et al. [1988] J. Neurosci. 8:4239-4261; Dann et al. [1987] Neurosci. Lett. 80:21-26; Dann et al. [1988] J. Neurosci. 8(5):1485-1499). The development of the remaining cells (about 50%), which constitute a heterogeneous group and are referred to here collectively as gamma cells (Boycott and Wässle, '74), has not been studied in detail. The purpose of this study was to compare the postnatal development of alpha, beta, and gamma cells in kitten and adult retinae using horseradish peroxidase histochemistry and the fluorescent dye DiI. In the kitten, alpha, beta, and gamma cells are recognizable. We find, as have others, that kitten alpha and beta cell bodies and dendritic fields are significantly smaller than in the adult. However, kitten gamma cells are nearly adult sized. In fact, at birth the cell bodies of beta cells throughout the retina are significantly smaller than those of gamma cells. During the first 12 weeks of life, alpha and beta cell bodies increase in size from 90% to 680% depending upon eccentricity. Gamma cells hardly increase in size at all. Also, the normal adult center-to-peripheral cell size gradient for alpha and beta cells is not seen in the neonate. Gamma cells show no such gradient in the neonate or adult. Our results suggest that the morphological development of alpha and beta cells occurs later than that of gamma cells and may explain some of the differences in the effects of visual deprivation and surgical manipulation upon the parallel Y-, X-, and W-cell pathways.
以往的研究人员已记录了猫视网膜中α型和β型神经节细胞的出生后发育情况（拉莫阿等人[1987年]《科学》237卷：522 - 525页；拉莫阿等人[1988年]《神经科学杂志》8卷：4239 - 4261页；丹恩等人[1987年]《神经科学快报》80卷：21 - 26页；丹恩等人[1988年]《神经科学杂志》8(5)卷：1485 - 1499页）。其余细胞（约占50%）构成一个异质群体，在这里统称为γ细胞（博伊科特和韦塞尔，1974年），其发育情况尚未得到详细研究。本研究的目的是使用辣根过氧化物酶组织化学和荧光染料碘化丙啶，比较幼猫和成年猫视网膜中α、β和γ细胞的出生后发育情况。在幼猫中，α、β和γ细胞是可识别的。我们和其他人一样发现，幼猫的α和β细胞体以及树突野明显小于成年猫。然而，幼猫的γ细胞大小已接近成年。实际上，出生时整个视网膜中β细胞的细胞体明显小于γ细胞。在出生后的前12周内，α和β细胞体的大小根据离心率从90%增加到680%。γ细胞几乎完全没有增大。此外，新生儿中未观察到成年时α和β细胞正常的中央到周边细胞大小梯度。γ细胞在新生儿或成年猫中均未显示出这种梯度。我们的结果表明，α和β细胞的形态发育比γ细胞晚，这可能解释了视觉剥夺和手术操作对平行的Y、X和W细胞通路的影响存在差异的部分原因。
