Vizzard M A, Erdman S L, Erickson V L, Stewart R J, Roppolo J R, De Groat W C
Department of Pharmacology, University of Pittsburgh, School of Medicine, Pennsylvania 15261.
J Comp Neurol. 1994 Jan 1;339(1):62-75. doi: 10.1002/cne.903390107.
The distribution of NADPH-d activity in the spinal cord and dorsal root ganglia of the cat was studied to evaluate the role of nitric oxide in lumbosacral afferent and spinal autonomic pathways. At all levels of the spinal cord NADPH-d staining was present in neurons and fibers in the superficial dorsal horn and in neurons around the central canal and in the dorsal commissure. In addition, the sympathetic autonomic nucleus in the rostral lumbar segments exhibited prominent NADPH-d cellular staining whereas the parasympathetic nucleus in the sacral segments was not well stained. The most prominent NADPH-d activity in the sacral segments occurred in fibers extending from Lissauer's tract through laminae I along the lateral edge of the dorsal horn to lamina V and the region of the sacral parasympathetic nucleus. These fibers were very similar to VIP-containing and pelvic nerve afferent projections in the same region. They were prominent in the S1-S3 segments but not in adjacent segments (L6-L7 and Cx1) or in thoracolumbar and cervical segments. NADPH-d activity and VIP immunoreactivity in Lissauer's tract and the lateral dorsal horn were eliminated or greatly reduced after dorsal-ventral rhizotomy (S1-S3), indicating the fibers represent primary afferent projections. A population of small diameter afferent neurons in the L7-S2 dorsal root ganglia were intensely stained for NADPH-d. The functional significance of the NADPH-d histochemical stain remains to be determined; however, if NADPH-d is nitric oxide synthase then this would suggest that nitric oxide may function as a transmitter in thoracolumbar sympathetic preganglionic efferent pathways and in sacral parasympathetic afferent pathways in the cat.
为评估一氧化氮在腰骶部传入神经和脊髓自主神经通路中的作用，研究了猫脊髓和背根神经节中NADPH-d活性的分布。在脊髓的所有节段，浅表背角的神经元和纤维、中央管周围的神经元以及背侧连合中均存在NADPH-d染色。此外，腰段上部的交感自主神经核显示出明显的NADPH-d细胞染色，而骶段的副交感神经核染色不佳。骶段最显著的NADPH-d活性出现在从Lissauer束延伸穿过I层沿背角外侧边缘至V层和骶副交感神经核区域的纤维中。这些纤维与同一区域含VIP的纤维和盆神经传入投射非常相似。它们在S1-S3节段中很突出，但在相邻节段（L6-L7和Cx1）以及胸腰段和颈段中不突出。在腹背根切断术（S1-S3）后，Lissauer束和背外侧角中的NADPH-d活性和VIP免疫反应性消失或大大降低，表明这些纤维代表初级传入投射。L7-S2背根神经节中的一群小直径传入神经元被强烈染成NADPH-d。NADPH-d组织化学染色的功能意义尚待确定；然而，如果NADPH-d是一氧化氮合酶，那么这表明一氧化氮可能在猫胸腰段交感神经节前传出通路和骶副交感神经传入通路中作为一种递质发挥作用。
