Schwartz E, Mezick J A, Gendimenico G J, Kligman L H
Department of Dermatology, University of Pennsylvania School of Medicine, Philadelphia 19104-6142.
J Invest Dermatol. 1994 Feb;102(2):241-6. doi: 10.1111/1523-1747.ep12371770.
In an earlier study we showed that tretinoin could prevent corticosteroid-induced skin atrophy in hairless mice. In this study, we examined the histochemical, biochemical, and immunochemical changes that accompanied the atrophy and its prevention. Mice were treated dorsally for 3 weeks in the morning and afternoon (AM:PM) as follows: 1) vehicle:vehicle, 2) steroid:vehicle, 3) steroid:tretinoin. Tretinoin concentration was 0.05% in an ethanol:propylene glycol vehicle. The steroid was clobetasol propionate (0.05%). The normally sparse dermal glycosaminoglycans were further reduced by steroid:vehicle treatment and increased to greater than vehicle:vehicle amounts by steroid:retinoid. Mast cells were similarly affected. Biochemical quantification of glycosaminoglycans confirmed the histochemical findings. Collagen, non-collagenous protein, and total protein content were reduced by the steroid. The latter two were returned to more normal levels by tretinoin whereas with collagen there was only a trend toward normal levels. Fibronectin, which was increased by the steroid:vehicle treatment, was reduced to more normal levels by steroid:tretinoin. We conclude that tretinoin has the ability to prevent the major steroid-induced biomechanical changes in hairless mouse dermal connective tissue that contribute to atrophy.
在一项早期研究中，我们发现维甲酸可预防无毛小鼠中皮质类固醇诱导的皮肤萎缩。在本研究中，我们检查了伴随萎缩及其预防过程的组织化学、生物化学和免疫化学变化。小鼠背部在上午和下午接受3周治疗，分组如下：1）赋形剂：赋形剂，2）类固醇：赋形剂，3）类固醇：维甲酸。维甲酸在乙醇：丙二醇赋形剂中的浓度为0.05%。类固醇为丙酸氯倍他索（0.05%）。正常情况下稀疏的真皮糖胺聚糖在类固醇：赋形剂治疗后进一步减少，而在类固醇：维甲酸治疗后增加至高于赋形剂：赋形剂组的水平。肥大细胞也受到类似影响。糖胺聚糖的生化定量证实了组织化学结果。类固醇使胶原蛋白、非胶原蛋白和总蛋白含量降低。后两者在维甲酸作用下恢复到更正常的水平，而胶原蛋白仅呈现出趋于正常水平的趋势。在类固醇：赋形剂治疗中增加的纤连蛋白，在类固醇：维甲酸治疗后降低至更正常的水平。我们得出结论，维甲酸有能力预防无毛小鼠真皮结缔组织中主要的类固醇诱导的生物力学变化，这些变化会导致萎缩。
