Fukushima O, Komiya M, Yamashita H
Department of Anatomy (I), Jikei University School of Medicine, Tokyo, Japan.
J Electron Microsc (Tokyo). 1993 Oct;42(5):351-5.
Cytochrome c oxidase activity in rat liver was demonstrated in 40 microns sections and ultrathin frozen sections (100-200 nm in thickness), using the diaminobenzidine method. Electron microscopic observations showed strong activity in 81.9 +/- 13.1% of mitochondria along the edge, and 29.9 +/- 21.2% in the center of 40 microns sections. On the other hand, all mitochondria possessed strong activity in ultrathin frozen sections under the same experimental condition. In the ultrathin frozen section, the mitochondrial membranes, as well as the plasma membrane, were broken. The present study indicates that the ultrathin frozen section is suitable for demonstrating cytochrome c oxidase activity at electron microscopic level.
采用二氨基联苯胺法，在大鼠肝脏的40微米切片和超薄冷冻切片（厚度为100 - 200纳米）中检测细胞色素c氧化酶活性。电子显微镜观察显示，在40微米切片边缘，81.9 +/- 13.1%的线粒体具有强活性，在切片中心为29.9 +/- 21.2%。另一方面，在相同实验条件下，超薄冷冻切片中的所有线粒体均具有强活性。在超薄冷冻切片中，线粒体膜以及质膜均已破裂。本研究表明，超薄冷冻切片适用于在电子显微镜水平上显示细胞色素c氧化酶活性。
