从断奶兔中分离出的致病性和非致病性大肠杆菌菌株中eaeA序列的存在情况。
Presence of eaeA sequences in pathogenic and nonpathogenic Escherichia coli strains isolated from weaned rabbits.
作者信息
Leroy S M, Lesage M C, Chaslus-Dancla E, Lafont J P
机构信息
Station de Pathologie Aviaire et de Parasitologie, Institut National de la Recherche Agronomique, Centre de Tours, Nouzilly, France.
出版信息
J Med Microbiol. 1994 Feb;40(2):90-4. doi: 10.1099/00222615-40-2-90.
Seventy-one Escherichia coli strains isolated from diarrhoeic weaned rabbits from different areas of France were tested for the presence of DNA sequences specific for the EPEC, EHEC, DAEC and EAggEC strains and 16 of them were tested for pathogenicity in animal experiments. High pathogenicity was observed only with strains unable to ferment rhamnose. DNA from all 55 rhamnose-negative O103, O26 and rough strains hybridised with the eaeA probe. Similar hybridisation was obtained with six non-pathogenic rhamnose-positive strains belonging to serogroups O128 and O132. No hybridisation was observed with the other probes. This is the first report of the presence of eaeA sequences in genomic DNA of non-pathogenic strains.
对从法国不同地区腹泻断奶兔中分离出的71株大肠杆菌菌株进行检测，以确定其是否存在针对肠致病性大肠杆菌（EPEC）、肠出血性大肠杆菌（EHEC）、弥漫性黏附性大肠杆菌（DAEC）和肠集聚性大肠杆菌（EAggEC）菌株的DNA序列，并对其中16株进行动物实验致病性检测。仅在不能发酵鼠李糖的菌株中观察到高致病性。所有55株鼠李糖阴性的O103、O26和粗糙型菌株的DNA与eaeA探针杂交。属于O128和O132血清型的6株非致病性鼠李糖阳性菌株也获得了类似的杂交结果。与其他探针未观察到杂交现象。这是关于非致病性菌株基因组DNA中存在eaeA序列的首次报道。
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