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用于分离耐甲氧西林金黄色葡萄球菌的三种半选择性培养基的比较

A comparison of three semi-selective media for the isolation of methicillin-resistant Staphylococcus aureus.

作者信息

Allen J L, Cowan M E, Cockroft P M

机构信息

Infection Control Department, St Mary's General Hospital, Portsmouth, Hants.

出版信息

J Med Microbiol. 1994 Feb;40(2):98-101. doi: 10.1099/00222615-40-2-98.

DOI:10.1099/00222615-40-2-98
PMID:8107069
Abstract

During the 1980s the emergence of multiple antibiotic-resistant strains of Staphylococcus aureus posed new problems for infection control worldwide, many of which still remain. In investigations of outbreaks of infection, the laboratory has a key role in identifying infected or colonised patients and staff. The rapid isolation and accurate identification of the causative organisms are essential for the implementation of appropriate control measures. Speed and accuracy in identification, by colonial morphology, is often difficult to achieve in the presence of a mixed population of commensal bacteria. To this end, the sensitivity of three media for the isolation of methicillin-resistant S. aureus (MRSA) from simulated clinical specimens was compared. Initial colonial recognition of MRSA was enhanced on methicillin-milk agar when compared with that on other media.

摘要

在20世纪80年代,耐多种抗生素的金黄色葡萄球菌菌株的出现给全球感染控制带来了新问题,其中许多问题至今仍然存在。在感染暴发调查中,实验室在识别感染或定植的患者及工作人员方面起着关键作用。快速分离和准确鉴定致病微生物对于实施适当的控制措施至关重要。在共生细菌混合菌群存在的情况下,通过菌落形态进行鉴定时,要实现速度和准确性往往很困难。为此,比较了三种培养基从模拟临床标本中分离耐甲氧西林金黄色葡萄球菌(MRSA)的敏感性。与其他培养基相比,在甲氧西林牛奶琼脂上对MRSA的初始菌落识别得到了增强。

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引用本文的文献

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Comparison of culture screening methods for detection of nasal carriage of methicillin-resistant Staphylococcus aureus: a prospective study comparing 32 methods.检测耐甲氧西林金黄色葡萄球菌鼻腔定植的培养筛查方法比较:一项比较32种方法的前瞻性研究
J Clin Microbiol. 2003 Jul;41(7):3163-6. doi: 10.1128/JCM.41.7.3163-3166.2003.
2
Soft salt-mannitol agar-cloxacillin test: a highly specific bedside screening test for detection of colonization with methicillin-resistant Staphylococcus aureus.软盐-甘露醇琼脂-氯唑西林试验:一种用于检测耐甲氧西林金黄色葡萄球菌定植的高特异性床旁筛查试验。
J Clin Microbiol. 1998 Apr;36(4):986-9. doi: 10.1128/JCM.36.4.986-989.1998.