Morandi M A, Del Rio J A, Caren R P, Caren L D
Department of Biology, California State University, Northridge 91330.
Life Sci. 1994;54(11):731-8. doi: 10.1016/0024-3205(94)90162-7.
The relationship between exposure to electromagnetic fields (EMF) and human health is of increasing interest. Exposure to EMF has been linked to leukemia and brain tumors in some but not all epidemiological studies. The effects of separate and combined alternating electric and magnetic fields on interleukin 1 (IL-1) and interleukin 6 (IL-6) production were measured in this study. Helmholtz coils and parallel plate electrodes were used to create uniform field characteristics (300 V/in., 0.3 mT). Effects were studied at a combined field frequency of 60 Hz. This frequency did not elevate culture temperatures above ambient room temperature. Murine thioglycollate-elicited peritoneal exudate cells (PEC) were exposed to an electric field (E), magnetic field (M), combined electric and magnetic field (EM), or no field (control). Three samples of PEC from each mouse were cultured with lipopolysaccharide in each field. Using commercial ELISA kits, supernatants of cell cultures were tested in duplicate after 24 hours of exposure for IL-1 alpha levels and after 48 hours of exposure for IL-6 levels. Results were evaluated using one-way analysis of variance (ANOVA). As a group, IL-1 production by the PEC from five mice and IL-6 production by the PEC from nine mice were unaffected by electric, magnetic, or combined electric and magnetic fields. Results from these experiments indicate that the 24-hour exposure to 60 Hz electric, magnetic, or combined electromagnetic fields had no effect on IL-1 production. Forty-eight hours of exposure to the same fields did not affect IL-6 production.
电磁场（EMF）暴露与人类健康之间的关系越来越受到关注。在一些但并非所有的流行病学研究中，电磁场暴露已与白血病和脑肿瘤相关联。本研究测量了单独和组合的交变电场和磁场对白细胞介素1（IL-1）和白细胞介素6（IL-6）产生的影响。使用亥姆霍兹线圈和平行板电极来创建均匀的场特性（300伏/英寸，0.3毫特斯拉）。在60赫兹的组合场频率下研究其影响。该频率不会使培养温度升高到环境室温以上。将小鼠巯基乙酸诱导的腹腔渗出细胞（PEC）暴露于电场（E）、磁场（M）、组合电场和磁场（EM）或无场（对照）。将每只小鼠的三个PEC样本在每个场中与脂多糖一起培养。使用商业ELISA试剂盒，在暴露24小时后对细胞培养上清液进行重复检测以测定IL-1α水平，在暴露48小时后检测IL-6水平。使用单因素方差分析（ANOVA）评估结果。总体而言，五只小鼠的PEC产生的IL-1和九只小鼠的PEC产生的IL-6不受电场、磁场或组合电场和磁场的影响。这些实验结果表明，24小时暴露于60赫兹的电场、磁场或组合电磁场对IL-1的产生没有影响。暴露于相同场48小时对IL-6的产生没有影响。
