Schoenmakers H C, van der Meulen-Muisers J J, Koornneef M
Department of Genetics, Wageningen Agricultural University, The Netherlands.
Mol Gen Genet. 1994 Feb;242(3):313-20. doi: 10.1007/BF00280421.
This paper describes the aggregation of nuclei in heterokaryons of tomato and unirradiated or irradiated potato protoplasts and the effects of gamma irradiation of potato and tomato protoplasts on single- and double-stranded DNA fragmentation, DNA repair and DNA synthesis as revealed by alkaline and pulsed field gel electrophoresis and an immunocytochemical technique. The prospects for obtaining highly asymmetric somatic hybrids of tomato and gamma-irradiated potato are discussed.
本文描述了番茄与未辐照或辐照过的马铃薯原生质体异核体中细胞核的聚集情况,以及马铃薯和番茄原生质体的γ射线辐照对单链和双链DNA片段化、DNA修复及DNA合成的影响,这些影响通过碱性和脉冲场凝胶电泳以及免疫细胞化学技术得以揭示。文中还讨论了获得番茄与γ射线辐照马铃薯的高度不对称体细胞杂种的前景。