Evtushenko D P, Shakhovskiĭ A M, Sidorov V A
Tsitol Genet. 2001 Nov-Dec;35(6):3-9.
Interspecific cybrid plants of potato were created using the cell technology that does not require the presence of any genetic selectable markers in the parental material. Cybrid production was based on double inactivation of S. pinnatisectum Dun. protoplasts, served as the donors of organelles (by gamma-irradiation for the nuclei inactivation and by chemical mutagenesis for the efficient induction of chlDNA mutations), and the transfer of mutant plastids into S. tuberosum L. by protoplast fusion. Selection of cell colonies for streptomycin resistance was performed to identify the cybrid clones with mutant donor-type plastids. Restriction analysis of organelle DNA, chromosome counting, and isoenzyme analysis of the cybrids revealed the presence of nuclear material of S. tuberosum L. (cv. Svitanok kyivsky) and plastids from wild tuber-bearing S. pinnatisectum Dun. These plants enable the study of traits encoded by organelle DNA and to broaden the cytoplasmic diversity of cultivated potato.
利用细胞技术培育出了马铃薯种间胞质杂种植物，该技术并不要求亲本材料中存在任何遗传选择标记。胞质杂种的产生基于对羽裂叶茄（S. pinnatisectum Dun.）原生质体的双重失活，羽裂叶茄原生质体作为细胞器供体（通过γ射线辐照使细胞核失活，通过化学诱变高效诱导叶绿体DNA突变），并通过原生质体融合将突变质体转移到马铃薯（S. tuberosum L.）中。通过对链霉素抗性的细胞集落进行筛选，以鉴定具有突变供体型质体的胞质杂种克隆。对胞质杂种的细胞器DNA进行限制性分析、染色体计数和同工酶分析，结果表明存在马铃薯（S. tuberosum L.，品种Svitanok kyivsky）的核物质以及来自野生块茎羽裂叶茄（S. pinnatisectum Dun.）的质体。这些植物有助于研究由细胞器DNA编码的性状，并拓宽栽培马铃薯的细胞质多样性。
