昆虫激素对体外培养的黑腹果蝇大规模分离的成虫盘RNA聚合酶的影响。
Effect of insect hormones on RNA polymerases of mass-isolated imaginal discs of Drosophila melanogaster cultured in vitro.
作者信息
Nishiura J T, Fristrom J W
出版信息
Proc Natl Acad Sci U S A. 1975 Aug;72(8):2984-8. doi: 10.1073/pnas.72.8.2984.
Four chromatographically separable DNA-dependent RNA polymerases (nucleosidetriphosphate:RNA nucleotidyltransferase; EC 2.7.7.6) were partially purified from imaginal discs of Drosophila melanogaster. Their properties are similar to those described for RNA polymerases I and II isolated from other eukaryotes. In vitro incubation of discs with beta-ecdysone, juvenile hormone, or cycloheximide resulted in increased activity of RNA polymerase I. The increase was irreversible with beta-ecdysone incubation and removal but reversible with juvenile hormone incubation and removal. With beta-ecdysone, the rate of the increase in polymerase I activity paralleled the kinetics of ecdysone binding to discs and increases in the rate of precursor incorporation into RNA. A model to explain the increased acticity of RNA polymerase I is presented.
从黑腹果蝇的成虫盘部分纯化出了四种可通过色谱分离的依赖DNA的RNA聚合酶（核苷三磷酸：RNA核苷酸转移酶；EC 2.7.7.6）。它们的特性与从其他真核生物中分离出的RNA聚合酶I和II的特性相似。用β-蜕皮激素、保幼激素或环己酰亚胺对成虫盘进行体外孵育，会导致RNA聚合酶I的活性增加。β-蜕皮激素孵育和去除后这种增加是不可逆的，但保幼激素孵育和去除后是可逆的。对于β-蜕皮激素，聚合酶I活性增加的速率与蜕皮激素与成虫盘结合的动力学以及前体掺入RNA的速率增加平行。本文提出了一个解释RNA聚合酶I活性增加的模型。
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