黑腹果蝇成虫盘内的蜕皮甾体受体
Ecdysteroid receptors in imaginal discs of Drosophila melanogaster.
作者信息
Yund M A, King D S, Fristrom J W
出版信息
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6039-43. doi: 10.1073/pnas.75.12.6039.
Abstract
[3H]Ponasterone A (PNA) of high specific activity has been used to identify and begin characterization of ecdysteroid (formerly called ecdysone) receptors in cytoplasmic and nuclear fractions of imaginal discs of Drosophila melanogaster. The equilibrium Kd of the observed macromolecular binding, 3--4 X 10(-9) M PNA, is in good agreement with the minimal concentration required for induction of complete morphogenesis in vitro, 4.2 X 10(-9) M PNA. Binding is analog specific and has kinetics consistent with a role in hormone response. On gentle homogenization, less than 5% of the binding capacity of the cell is released as soluble receptor; the other 95% remains with the nuclear fraction. This nuclear fraction specifically binds [3H]PNA in vitro. Greater than 95% of nuclear PNA receptors are released by extraction with 0.3 M KCl. The binding properties of the nuclear receptors are indistinguishable from those of the cytosol fraction or of the whole cell.
摘要
高比活度的[3H]脱皮甾酮A（PNA）已被用于鉴定和初步表征黑腹果蝇成虫盘细胞质和细胞核组分中的蜕皮甾体（以前称为蜕皮激素）受体。观察到的大分子结合的平衡解离常数Kd为3 - 4×10^(-9) M PNA，与体外诱导完全形态发生所需的最低浓度4.2×10^(-9) M PNA高度一致。结合具有类似物特异性，其动力学与在激素反应中的作用一致。在温和匀浆时，细胞结合能力的不到5%以可溶性受体形式释放；其余95%仍与细胞核组分结合。该细胞核组分在体外特异性结合[3H]PNA。超过95%的细胞核PNA受体可通过用0.3 M KCl提取而释放。细胞核受体的结合特性与细胞质组分或整个细胞的结合特性没有区别。
相似文献
1
Ecdysteroid receptors in imaginal discs of Drosophila melanogaster.黑腹果蝇成虫盘内的蜕皮甾体受体
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6039-43. doi: 10.1073/pnas.75.12.6039.
2
Demonstration of an ecdysteroid receptor in a cultured cell line of Drosophila melanogaster.黑腹果蝇培养细胞系中蜕皮甾体受体的证明。
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6035-8. doi: 10.1073/pnas.75.12.6035.
3
The uptake and release of ponasterone A by the Kc cell line of Drosophila melanogaster.黑腹果蝇Kc细胞系对蜕皮甾酮A的摄取与释放
Mol Cell Endocrinol. 1980 Jan;17(1):51-9. doi: 10.1016/0303-7207(80)90103-3.
4
Specific binding of 20-hydroxyecdysone to nuclei of imaginal discs of Drosophila melanogaster.20-羟基蜕皮激素与黑腹果蝇成虫盘细胞核的特异性结合。
Mol Cell Endocrinol. 1979 Apr;14(1):19-35. doi: 10.1016/0303-7207(79)90055-8.
5
Characterization of ecdysteroid receptors in cytosol and naive nuclear preparations of Drosophila Kc cells.
J Biol Chem. 1982 Jun 10;257(11):6373-9.
6
26-[125I]iodoponasterone A is a potent ecdysone and a sensitive radioligand for ecdysone receptors.26-[125I]碘蜕皮甾酮A是一种强效蜕皮激素，也是蜕皮激素受体的敏感放射性配体。
Proc Natl Acad Sci U S A. 1988 Apr;85(7):2096-100. doi: 10.1073/pnas.85.7.2096.
7
Characterization of cytoplasmic ecdysteroid receptors in the hypodermis of the crayfish, Orconectes limosus.
Mol Cell Endocrinol. 1981 Dec;24(3):253-65. doi: 10.1016/0303-7207(81)90002-2.
8
Affinity labelling of a partially purified ecdysteroid receptor with a bromoacetylated 20-OH-ecdysone derivative.
Eur J Biochem. 1990 Apr 20;189(1):137-43. doi: 10.1111/j.1432-1033.1990.tb15470.x.
9
A reappraisal of ecdysteroid binding in Drosophila.
Mol Cell Endocrinol. 1983 Oct;32(2-3):171-8. doi: 10.1016/0303-7207(83)90080-1.
10
Heterodimerization of the Drosophila ecdysone receptor with retinoid X receptor and ultraspiracle.果蝇蜕皮激素受体与视黄酸X受体和超气门蛋白的异源二聚化。
Nature. 1993 Apr 1;362(6419):471-5. doi: 10.1038/362471a0.
引用本文的文献
1
The morphological response of Kc-H cells to ecdysteroids: Hormonal specificity.Kc-H细胞对蜕皮甾体的形态学反应：激素特异性。
Wilehm Roux Arch Dev Biol. 1980 Feb;189(1):1-15. doi: 10.1007/BF00848562.
2
Changes in cell surface proteins of culturedDrosophila cells exposed to 20-hydroxyecdysone.暴露于20-羟基蜕皮激素的培养果蝇细胞的细胞表面蛋白变化。
Wilehm Roux Arch Dev Biol. 1983 Mar;192(2):103-107. doi: 10.1007/BF00848486.
3
Imaginal disc development in a non-pupariating lethal mutant inDrosophila melanogaster.黑腹果蝇非蛹化致死突变体中的成虫盘发育
Wilehm Roux Arch Dev Biol. 1981 Jan;190(1):11-21. doi: 10.1007/BF00868698.
4
Identification and characterization of the ecdysterone receptor in Drosophila melanogaster by photoaffinity labeling.利用光亲和标记鉴定和表征黑腹果蝇中的蜕皮激素受体。
Proc Natl Acad Sci U S A. 1982 Jan;79(1):6-10. doi: 10.1073/pnas.79.1.6.
5
Juvenile hormone-binding protein from the cytosol of Drosophila Kc cells.果蝇 Kc 细胞细胞质中的保幼激素结合蛋白。
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4657-61. doi: 10.1073/pnas.77.8.4657.
6
Molecular determinants of differential ligand sensitivities of insect ecdysteroid receptors.昆虫蜕皮甾体受体不同配体敏感性的分子决定因素。
Mol Cell Biol. 2000 Jun;20(11):3870-9. doi: 10.1128/MCB.20.11.3870-3879.2000.
7
The effects of 20-hydroxyecdysone on the differentiation in vitro of cells from the eye imaginal disc from Drosophila melanogaster.20-羟基蜕皮激素对黑腹果蝇眼成虫盘细胞体外分化的影响。
Invert Neurosci. 1997 Jun;3(1):57-69. doi: 10.1007/BF02481715.
8
Cytodifferentiation of the accessory glands of Tenebrio molitor. IX. differentiation of the spermathecal accessory gland in vitro.黄粉虫附腺的细胞分化。IX. 受精囊附腺的体外分化
Cell Tissue Res. 1982;222(2):269-81. doi: 10.1007/BF00213212.
9
Photoinduced bonding of endogenous ecdysterone to salivary gland chromosomes of Chironomus tentans.光诱导内源性蜕皮激素与摇蚊唾腺染色体的结合。
Chromosoma. 1981;82(4):543-59. doi: 10.1007/BF00295012.
10
Localization of ecdysterone on polytene chromosomes of Drosophila melanogaster.蜕皮甾酮在黑腹果蝇多线染色体上的定位
Proc Natl Acad Sci U S A. 1980 Apr;77(4):2108-12. doi: 10.1073/pnas.77.4.2108.
本文引用的文献
1
The Differentiation of Eye Pigments in Drosophila as Studied by Transplantation.通过移植研究果蝇眼部色素的分化
Genetics. 1936 May;21(3):225-47. doi: 10.1093/genetics/21.3.225.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Maintenance of imaginal discs of Drosophila melanogaster in chemically defined media.在化学成分明确的培养基中维持黑腹果蝇成虫盘。
J Cell Biol. 1969 Jun;41(3):876-85. doi: 10.1083/jcb.41.3.876.
4
The in vitro synthesis and characteristics of ribosomal RNA in imaginal discs of Drosophila melanogaster.黑腹果蝇成虫盘核糖体RNA的体外合成及特性
Mol Gen Genet. 1971;110(3):245-62. doi: 10.1007/BF00337837.
5
A new assessment of subcellular distribution of bound estrogen in the uterus.子宫中结合雌激素亚细胞分布的新评估。
Biochem Biophys Res Commun. 1971 Oct 1;45(1):258-64. doi: 10.1016/0006-291x(71)90078-7.
6
A simple and general extraction procedure for phytoecdysones based on reversed-phase adsorption chromatography.
Steroids. 1972 Mar;19(3):377-83. doi: 10.1016/0039-128x(72)90077-3.
7
Effects of -ecdysone on RNA metabolism of imaginal disks of Drosophila melanogaster.β-蜕皮激素对黑腹果蝇成虫盘RNA代谢的影响。
J Insect Physiol. 1971 Sep;17(9):1599-614. doi: 10.1016/0022-1910(71)90056-4.
8
An improved scintillation cocktail of high-solubilizing power.一种具有高增溶能力的改良闪烁液。
Anal Biochem. 1973 Jan;51(1):173-9. doi: 10.1016/0003-2697(73)90465-x.
9
The assay of ecdysones and juvenile hormones on Drosophila imaginal disks in vitro.果蝇成虫盘体外蜕皮激素和保幼激素的测定
J Insect Physiol. 1972 Jun;18(6):1115-23. doi: 10.1016/0022-1910(72)90146-1.
10
The effect of beta-ecdysone on protein synthesis in imaginal discs of Drosophila melanogaster cultured in vitro.β-蜕皮激素对体外培养的黑腹果蝇成虫盘蛋白质合成的影响。
Dev Biol. 1974 Dec;41(2):314-30. doi: 10.1016/0012-1606(74)90309-1.
Zotero 插件