Feo S, Di Liegro C, Jones T, Read M, Fried M
Istituto di Biologia dello Sviluppo C.N.R., Palermo, Italy.
Oncogene. 1994 Mar;9(3):955-61.
In order to study amplicons containing the c-myc locus in human tumour cells we have analysed the region around the human c-myc gene. Using pulsed field gel electrophoresis we have constructed a 1.8 Mb map of the c-myc locus using six rare-cutting endonucleases. In addition we have cloned 130 kb of continuous DNA around the c-myc gene which includes 65 kb of 5' sequence and 65 kb of 3' sequence. The first exon of the pvt-1 gene was located 59 kb 3' of the c-myc gene. Using nine neighbouring probes, covering a region of about 395 kb around the c-myc gene, we were able to determine the extent and composition of c-myc amplicons in the HL60, NCI-N417, Colo320HSR, SkBr3 and the Sk-N-MC human tumour cell lines. Furthermore we analysed the chromosomal location of amplified c-myc DNA in NCI-N417 and SkBr3 cells. Similarities and differences between the amplified DNA in the different cell lines are discussed.
为了研究人类肿瘤细胞中包含c-myc基因座的扩增子，我们分析了人类c-myc基因周围的区域。利用脉冲场凝胶电泳，我们使用六种稀有切割内切酶构建了c-myc基因座的1.8 Mb图谱。此外，我们克隆了c-myc基因周围130 kb的连续DNA，其中包括65 kb的5'序列和65 kb的3'序列。pvt-1基因的第一个外显子位于c-myc基因下游59 kb处。使用九个相邻探针，覆盖c-myc基因周围约395 kb的区域，我们能够确定HL60、NCI-N417、Colo320HSR、SkBr3和Sk-N-MC人类肿瘤细胞系中c-myc扩增子的范围和组成。此外，我们分析了NCI-N417和SkBr3细胞中扩增的c-myc DNA的染色体定位。讨论了不同细胞系中扩增DNA的异同。
