Ernst L M, Salafia C M, Carter A M, Pezzullo J C
Department of Pathology, University of Connecticut School of Medicine, Farmington 06302.
Pediatr Pathol. 1993 Nov-Dec;13(6):763-72. doi: 10.3109/15513819309048263.
Hepatic histology was examined in guinea pig fetuses in which intrauterine growth retardation (IUGR) was induced by unilateral uterine artery ligation and compared with that of control (well-grown) fetuses from uterine horns with intact circulations. From all animals, sections taken from the six lobes of the liver were prepared using hematoxylin and eosin stains. Periodic acid-Schiff and Prussian blue stains (both on fixed samples) and Oil Red O stains on frozen tissues were performed on a subset of samples. Comparisons revealed greater fat content in hepatocytes (P < .05) and decreased total hepatic hematopoiesis (P < .01) in IUGR animals than in controls. No differences in hepatocyte histology among lobes were observed in control animals. IUGR animals had increased fat content and decreased extramedullary hematopoiesis in the lobes receiving the portal circulation (P < .05). These data indicate significant abnormalities of hepatic lipid metabolism in IUGR animals following uterine artery ligation. An unexpected and counterintuitive finding was the decrease in hepatic hematopoiesis in the right side of the liver.
在通过单侧子宫动脉结扎诱导宫内生长受限（IUGR）的豚鼠胎儿中检查肝脏组织学，并与来自循环完整子宫角的对照（生长良好）胎儿的肝脏组织学进行比较。从所有动物中，使用苏木精和伊红染色制备取自肝脏六个叶的切片。对一部分样本进行了过碘酸-希夫染色和普鲁士蓝染色（均针对固定样本）以及对冷冻组织进行油红O染色。比较结果显示，与对照相比，IUGR动物的肝细胞脂肪含量更高（P < .05），肝脏总造血功能降低（P < .01）。在对照动物中，各叶肝细胞组织学未观察到差异。IUGR动物接受门静脉循环的叶中脂肪含量增加，髓外造血减少（P < .05）。这些数据表明子宫动脉结扎后IUGR动物肝脏脂质代谢存在明显异常。一个意外且与直觉相反的发现是肝脏右侧的肝脏造血功能下降。
