Peroutka M
Vet Med (Praha). 1975 Jun;20(7):373-84.
By counting the spores of protozoon Nosema apis Z. in Bürker's chamber the author was able to find, in 1495 caged bees sacrificed one week after the parasite invasion, from altogether 26 samples of various feeds statistically sifnificant differences of influencing the protozoon development only in sallow pollen. The differences between the individual feeds were statistically more significant in bees sacrificed 14 days after the invasion. Examination of 1260 bees 14 days after the invasion demonstrated that, as compared with glycide food, the parasite development was enhanced by a feed consisting of 6, 9, and 12 per cent fresh rape pollen, 3 and 6 per cent of fresh and dried sallow pollen, 6 per cent freeze-dried pollen mixture, pollen deposited in honeycombs, 7.5, 10, and 12.5 per cent yeast dough, "Arnika" and 3 per cent Bacto peptone. Even 14 days after the invasion no statistically sifnificant differences of influencing the schizogony of protozoon Nosema apis Z. could be demonstrated in bees fed pure glycide feed, dough with 3 and 6 per cent casein hydrolyzate, 3 and 6 per cent Hammarsten casein, 3 per cent dried whole eggs, 3 per cent rape pollen and 3 and 6 per cent fresh pollen mixtures. In the study the possibility of an indirect effect of pollen on the parasite schizogony through the secrete of the pharyngeal glands is being discussed.
通过在 Bürker 计数室中对蜜蜂微孢子虫 Nosema apis Z. 的孢子进行计数，作者发现，在寄生虫入侵一周后处死的 1495 只笼养蜜蜂中，从总共 26 种不同饲料样本来看，仅在柳树花粉中对原生动物发育有统计学上显著的影响差异。在入侵 14 天后处死的蜜蜂中，各饲料之间的差异在统计学上更为显著。对入侵 14 天后的 1260 只蜜蜂进行检查表明，与糖类食物相比，由 6%、9%和 12%的新鲜油菜花粉、3%和 6%的新鲜和干燥柳树花粉、6%的冻干花粉混合物、蜂巢中储存的花粉、7.5%、10%和 12.5%的酵母面团、“山金车”和 3%的细菌蛋白胨组成的饲料会促进寄生虫的发育。即使在入侵 14 天后，在喂食纯糖类饲料、含有 3%和 6%酪蛋白水解物的面团、3%和 6%哈马斯坦酪蛋白、3%的干全蛋、3%的油菜花粉以及 3%和 6%的新鲜花粉混合物的蜜蜂中，也未发现对蜜蜂微孢子虫 Nosema apis Z. 裂殖生殖有统计学上显著的影响差异。在该研究中，正在讨论花粉通过咽腺分泌物对寄生虫裂殖生殖产生间接影响的可能性。
