Schmidt H, Ebeling H, Kraft D
Z Klin Chem Klin Biochem. 1975 Mar;13(3):117-21.
Radial immunodiffusion, which is a presently accepted reference method, was compared with the mechanized immunoprecipitation. Each of these specific methods was compared with two normal clinical-chemical methods for the determination of albumin and transferrin, and an enzymic method for the determination of ceruloplasmin. The correlation coefficients, the mean slope for regression, and the percentage variation of the average values from those of the average values of the reference methods are presented. In the determination of albumin and ceruloplasmin, radial immunodiffusion and mechanized immunoprecipitation give the same results. Surprisingly, these two specific methods give different results in the determination of transferrin. In as far as there is no doubt concerning the accuracy of the mechanized immunoprecipitation, this method is recommended, since it also has the advantage of being less demanding in cost and time.
目前公认的参考方法——放射免疫扩散法，与机械化免疫沉淀法进行了比较。将这些特定方法中的每一种与两种常规临床化学方法进行比较，以测定白蛋白和转铁蛋白，并用一种酶法测定铜蓝蛋白。给出了相关系数、回归平均斜率以及平均值与参考方法平均值的百分比变化。在测定白蛋白和铜蓝蛋白时，放射免疫扩散法和机械化免疫沉淀法得出相同结果。令人惊讶的是，这两种特定方法在测定转铁蛋白时给出了不同结果。鉴于机械化免疫沉淀法的准确性毋庸置疑，推荐使用该方法，因为它在成本和时间方面要求较低。
