胰岛素依赖型糖尿病中的红细胞阳离子转运系统与膜脂

Erythrocyte cation transport systems and membrane lipids in insulin-dependent diabetes.

作者信息

Lijnen P, Fenyvesi A, Bex M, Bouillon R, Amery A

机构信息

Hypertension and Cardiovascular Rehabilitation Unit, University of Leuven, Belgium.

出版信息

Am J Hypertens. 1993 Sep;6(9):763-70. doi: 10.1093/ajh/6.9.763.

DOI:10.1093/ajh/6.9.763

PMID:8110430

Abstract

The relationship between erythrocyte cation transport systems and membrane and plasma lipids was examined in normal men and patients with insulin-dependent diabetes mellitus (IDDM). Different measurements of erythrocyte transport systems were obtained in patients with IDDM and in age- and weight-matched healthy men: Na+:Li+ countertransport activity or Li(+)-stimulated Na+ efflux, Na+:K+ cotransport activity or bumetanide-sensitive Na+ efflux, Na+:K+ pump activity or ouabain-sensitive Na+ efflux, and the ouabain- and bumetanide-resistant Na+ and K+ fluxes or the ground membrane permeability for Na+ and K+ as well as the intraerythrocyte Na+, K+, and Mg2+ concentrations. Plasma cholesterol, triglycerides, phospholipids, low- and high- density lipoprotein cholesterol, and erythrocyte membrane cholesterol and phospholipid content were obtained from the fasting subjects. The patients with IDDM had an elevated (P < .05 or less) erythrocyte Na+:Li+ countertransport activity, ground membrane leak for K+, intraerythrocyte K+ concentration, and erythrocyte membrane cholesterol content, but a lower red blood cell phospholipid content. In single regression analysis, the erythrocyte Na+:Li+ countertransport, Na+:K+ cotransport, and Na+:K+ pump activity and ground membrane leak for Na+ and K+ were inversely related to the red blood cell membrane lipid content. Our data in patients with IDDM show that a decreased erythrocyte membrane lipid content was accompanied by a higher erythrocyte Na+:Li+ countertransport, Na+:K+ cotransport, and Na+:K+ pump activity.

摘要

在正常男性和胰岛素依赖型糖尿病（IDDM）患者中，研究了红细胞阳离子转运系统与膜脂质和血浆脂质之间的关系。对IDDM患者以及年龄和体重匹配的健康男性进行了不同的红细胞转运系统测量：Na⁺:Li⁺逆向转运活性或Li⁺刺激的Na⁺外流、Na⁺:K⁺协同转运活性或布美他尼敏感的Na⁺外流、Na⁺:K⁺泵活性或哇巴因敏感的Na⁺外流，以及哇巴因和布美他尼耐药的Na⁺和K⁺通量或Na⁺和K⁺的基膜通透性，还有红细胞内Na⁺、K⁺和Mg²⁺浓度。从空腹受试者中获取血浆胆固醇、甘油三酯、磷脂、低密度和高密度脂蛋白胆固醇，以及红细胞膜胆固醇和磷脂含量。IDDM患者的红细胞Na⁺:Li⁺逆向转运活性、K⁺的基膜渗漏、红细胞内K⁺浓度和红细胞膜胆固醇含量升高（P <.05或更低），但红细胞磷脂含量较低。在单因素回归分析中，红细胞Na⁺:Li⁺逆向转运、Na⁺:K⁺协同转运和Na⁺:K⁺泵活性以及Na⁺和K⁺的基膜渗漏与红细胞膜脂质含量呈负相关。我们在IDDM患者中的数据表明，红细胞膜脂质含量降低伴随着较高的红细胞Na⁺:Li⁺逆向转运、Na⁺:K⁺协同转运和Na⁺:K⁺泵活性。