Lijnen P, Fagard R, Staessen J, Thijs L, Amery A
Department of Pathophysiology, University of Leuven, Belgium.
J Hypertens. 1992 Oct;10(10):1205-11. doi: 10.1097/00004872-199210000-00014.
The relationship between erythrocyte membrane and plasma lipids and various transmembrane erythrocyte cationic fluxes was examined in 53 normal men.
Different measurements of erythrocyte transport systems were obtained: Na(+)-Li+ countertransport activity; Na+, K+ cotransport activity; Na+, K(+)-ATPase pump activity and the ground membrane permeability for Na+ and K+ as well as the intra-erythrocyte Na+, K+ and Mg2+ concentrations. Plasma cholesterol, triglycerides, phospholipids, free fatty acids, low- and high-density lipoprotein cholesterol levels and the erythrocyte membrane contents of cholesterol, phospholipids and free fatty acids were obtained from fasting subjects.
In single regression analysis the erythrocyte Na(+)-Li+ countertransport and Na+, K+ cotransport activities were negatively related to the erythrocyte membrane cholesterol, phospholipids and free fatty acids contents. The Na+, K(+)-ATPase pump activity as assessed by the ouabain-sensitive Na+ efflux was also inversely related to the membrane cholesterol and phospholipids contents. In multiple regression analysis the red blood cell Na(+)-Li+ countertransport activity was independently and negatively related to the membrane cholesterol and free fatty acids contents.
Our data show that an elevated level of erythrocyte membrane lipids in normal men is accompanied by lower Na(+)-Li+ countertransport, Na+, K+ cotransport and Na+, K(+)-ATPase pump activities.
在53名正常男性中研究红细胞膜与血浆脂质及各种跨膜红细胞阳离子通量之间的关系。
获得了红细胞转运系统的不同测量值：钠-锂逆向转运活性；钠、钾协同转运活性；钠、钾-ATP酶泵活性以及钠和钾的基础膜通透性以及红细胞内钠、钾和镁离子浓度。从空腹受试者中获取血浆胆固醇、甘油三酯、磷脂、游离脂肪酸、低密度和高密度脂蛋白胆固醇水平以及红细胞膜胆固醇、磷脂和游离脂肪酸含量。
在单因素回归分析中，红细胞钠-锂逆向转运和钠、钾协同转运活性与红细胞膜胆固醇、磷脂和游离脂肪酸含量呈负相关。用哇巴因敏感的钠外流评估的钠、钾-ATP酶泵活性也与膜胆固醇和磷脂含量呈负相关。在多因素回归分析中，红细胞钠-锂逆向转运活性与膜胆固醇和游离脂肪酸含量独立且呈负相关。
我们的数据表明，正常男性红细胞膜脂质水平升高伴随着较低的钠-锂逆向转运、钠、钾协同转运和钠、钾-ATP酶泵活性。
