通过亲和层析法分离猪分泌型免疫球蛋白A及其组成链的测定
Isolation of porcine secretory immunoglobulin A by affinity chromatography and determination of its component chains.
作者信息
De Buysscher E V, Berman D T
出版信息
Am J Vet Res. 1975 Nov;36(11):1659-61.
Porcine secretory immunoglobulin A (SIgA) was isolated in a single step from porcine milk whey by affinity chromatography, using antiporcine alpha-chain specific antibody linked to CNBr-activated agarose. The isolated SIgA contained no other proteins detectable by immunodiffusion of immunoelectrophoresis. After reduction and alkylation with dithiothreitol and iodoacetamide, the SIgA was chromatographed on gel equilibrated with 6.0 M urea and 1.0 M propionic acid. Polyacrylamide gel electrohoresis of the fractions eluted revealed the presence of light chain, alpha-chain secretory component, and J-chain.
通过亲和色谱法，使用与溴化氰活化琼脂糖偶联的抗猪α链特异性抗体，从猪乳清中一步分离出猪分泌型免疫球蛋白A（SIgA）。通过免疫扩散或免疫电泳检测，分离出的SIgA不含其他蛋白质。用二硫苏糖醇和碘乙酰胺进行还原和烷基化后，将SIgA在含有6.0 M尿素和1.0 M丙酸的平衡凝胶上进行色谱分析。对洗脱的组分进行聚丙烯酰胺凝胶电泳，结果显示存在轻链、α链分泌成分和J链。
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