Powell L W, Alpert E, Isselbacher K J, Drysdale J W
Br J Haematol. 1975 May;30(1):47-55. doi: 10.1111/j.1365-2141.1975.tb00516.x.
Ferritins from human liver, spleen, heart, pancreas and kidney were compared by electrophoresis and isoelectric focusing in polyacrylamide gels, by immunodiffusion against antisera to homologous and heterologous ferritin, and in some cases by their cyanogen bromide peptides. All ferritins appeared to consist of a single species on gel electrophoresis with the exception of heart ferritin which separated into two major components. Small differences in electrophoretic mobility were found in all tissue ferritins. By contrast, all tissue ferritins were found to consist of multiple forms when analysed by gel electrofocusing. At least five isoferritins were found in most tissues, several of which were common to most tissues. At least two were common to all tissues. Those ferritins which were most easily distinguishable electrophoretically, e.g. spleen and heart ferritin, showed the greatest differences on gel electrofocusing. The ferritin profile was characteristic of each organ and was reproducible both within individuals and between individual tissues. There were striking differences in the iron content of the various isoferritins within a tissue. Further, the iron content of isoferritins common to more than one tissue varied with the tissue of origin. Some isoferritins in several organs and all of the isoferritins in pancreas appeared to contain little, if any, iron. All five tissue ferritins contained antigenic determinants in common with liver ferritin. However, an additional antigenic determinant was found in liver ferritin which was not detectable in the ferritins from the other organs.
通过聚丙烯酰胺凝胶电泳和等电聚焦、针对同源和异源铁蛋白抗血清的免疫扩散以及在某些情况下通过其溴化氰肽，对来自人肝脏、脾脏、心脏、胰腺和肾脏的铁蛋白进行了比较。除心脏铁蛋白分离为两个主要成分外，所有铁蛋白在凝胶电泳上似乎都由单一物种组成。在所有组织铁蛋白中发现电泳迁移率存在微小差异。相比之下，当通过凝胶电聚焦分析时，发现所有组织铁蛋白都由多种形式组成。在大多数组织中发现至少五种异铁蛋白，其中几种在大多数组织中是常见的。所有组织中至少有两种是常见的。那些在电泳上最容易区分的铁蛋白，例如脾脏和心脏铁蛋白，在凝胶电聚焦上显示出最大的差异。铁蛋白谱是每个器官的特征，在个体内部和个体组织之间都是可重复的。组织内各种异铁蛋白的铁含量存在显著差异。此外，一种以上组织共有的异铁蛋白的铁含量随其来源组织而变化。几个器官中的一些异铁蛋白以及胰腺中的所有异铁蛋白似乎几乎不含铁（如果含铁的话）。所有五种组织铁蛋白都含有与肝脏铁蛋白共有的抗原决定簇。然而，在肝脏铁蛋白中发现了一种额外的抗原决定簇，在其他器官的铁蛋白中无法检测到。
