Ikeda E, Yoshida S, Mitsuzawa H, Uno I, Toh-e A
Department of Biology, Faculty of Science, University of Tokyo, Japan.
FEBS Lett. 1994 Feb 21;339(3):265-8. doi: 10.1016/0014-5793(94)80428-1.
The grpE gene is a heat shock gene of Escherichia coli whose product functions as a chaperone to (re)fold proteins. We found a yeast homologue of grpE and designated it YGE1. YGE1 can replace grpE in E. coli, indicating that YGE1 is a functional homologue of grpE. Deletion of YGE1 is lethal. During depletion of the Yge1 product, mitochondria are sequestered in mother cells thereby accumulating cells without mitochondria, suggesting that Yge1 protein plays a pivotal role in maintaining mitochondrial functions.
grpE基因是大肠杆菌的一种热休克基因，其产物作为伴侣蛋白发挥作用，帮助蛋白质（重新）折叠。我们发现了grpE的一个酵母同源物，并将其命名为YGE1。YGE1可以替代大肠杆菌中的grpE，这表明YGE1是grpE的功能同源物。YGE1的缺失是致死性的。在Yge1产物缺失期间，线粒体被隔离在母细胞中，从而积累了没有线粒体的细胞，这表明Yge1蛋白在维持线粒体功能中起关键作用。
