Mileva Zh, Santé M C, Fusard C, Ruff F, Paro J L
Eksp Med Morfol. 1978;17(3):121-6.
The authors examined the changes in the histamine level, DAO (diaminoxidase) activity and N--MT (N-methyltransferase) activity in blood (plasma) and tissues of lung and liver (N--MT was examined only in the indicated tissues) in 105 guinea pigs, 45 of whom were healthy and 60 sensibilised and examined during the anaphylactic shock. It was established that during the acute anaphylactic shock there was demonstrative hyperhistanemia and hyperhistaminasemia, which were not observed during experimental anaphylactic shock with subacute course. The activity of lung and liver N--MT in animals with acute and subacute anaphylactic shock did not differ from that of the healthy guinea pigs. The complete inhibition of DAO activity in healthy animals and those with anaphylactic shock did not induce change in the activity of N--MT as well. The latter did not alter after sharp or high level or moderate elevation of the tissue histamine for several days. This histamine was a specific substrate for this enzyme. N--MT is an inductive enzyme, which apparently does not participate in histamine metabolism during acute and subacute anaphylaxis in guinea pigs. For its change, probably, is necessary longer time.
作者检测了105只豚鼠血液（血浆）以及肺和肝组织中组胺水平、DAO（二胺氧化酶）活性和N - MT（N - 甲基转移酶）活性的变化（仅在指定组织中检测N - MT），其中45只为健康豚鼠，60只为致敏豚鼠，并在过敏性休克期间进行检测。结果发现，在急性过敏性休克期间出现了明显的高组胺血症和高组胺酶血症，而在亚急性病程的实验性过敏性休克期间未观察到这种情况。急性和亚急性过敏性休克动物的肺和肝N - MT活性与健康豚鼠的活性没有差异。健康动物和过敏性休克动物中DAO活性的完全抑制也未引起N - MT活性的变化。在组织组胺急剧升高、高水平或中度升高数天后，N - MT活性也没有改变。这种组胺是该酶的特异性底物。N - MT是一种诱导酶，在豚鼠急性和亚急性过敏反应期间显然不参与组胺代谢。其变化可能需要更长时间。
