Howes K A, Lasudry J G, Albert D M, Windle J J
Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio.
Invest Ophthalmol Vis Sci. 1994 Feb;35(2):342-51.
To produce transgenic mice that express the SV40 T-antigen oncogene specifically in photoreceptor cells, giving rise to retinoblastoma tumors of photoreceptor cell origin; to characterize the mice with regard to transgene expression and pathology and to characterize the resulting tumors histologically.
Transgenic mice were generated that express T-antigen under the control of the murine interstitial retinol binding protein promoter.
All mice produced developed either ocular or intracranial tumors, or both, at an early age. One line of mice was generated, and all mice of this line develop both retinal photoreceptor cell and pineal tumors by as early as 2 weeks of age. Cell lines have been established from both tumor types.
These mice represent an animal model system for human trilateral retinoblastoma, in which retinoblastomas are accompanied by pineal tumors.
培育在光感受器细胞中特异性表达SV40 T抗原癌基因的转基因小鼠，从而引发源自光感受器细胞的视网膜母细胞瘤；从转基因表达和病理学方面对小鼠进行特征描述，并对所产生的肿瘤进行组织学特征描述。
培育在小鼠间质视黄醇结合蛋白启动子控制下表达T抗原的转基因小鼠。
所有产生的小鼠在早期都发生了眼部或颅内肿瘤，或两者皆有。培育出了一个品系的小鼠，该品系的所有小鼠早在2周龄时就同时发生视网膜光感受器细胞肿瘤和松果体肿瘤。已从这两种肿瘤类型中建立了细胞系。
这些小鼠代表了人类三边性视网膜母细胞瘤的动物模型系统，其中视网膜母细胞瘤伴有松果体肿瘤。
