Ashton F E, Charron F, Ota F, Wallace R, Diena B B
Can J Microbiol. 1975 Nov;21(11):1751-5. doi: 10.1139/m75-255.
Growth of Neisseria gonorrhoeae in the presence of Rhesus monkey kidney (RE2) cells containing Eagle's minimal essential medium (MEM) 'conditioned' by 18-20 h of contact with RE2 cells began 3-4 h after inoculation and by 16-20 h had reached a maximum. When 'conditioned' medium (CM) was removed from the tissue cells and replaced with fresh medium a 5-6 h lag period occurred before multiplication of gonococci began and by 16 h growth was also near a maximum. CM removed from tissue cells supported the growth of six strains of N. gonorrhoeae. Three strains failed to grow in CM but multiplied when tissue cells were present. None of the strains grew in the presence of tissue cells killed by heating or in tissue-culture medium alone. Of the six strains of N. gonorrhoeae which grew in CM, three kept as their major (90%) population T1 colonies, while the remaining strains produced T1 organisms and T1A, T3, and T4 colonial variants. These results indicate that tissue cells secrete growth factors which support the growth and maintenance of T1 colonial forms of certain strains of N. gonnorhoeae.
在含有经与恒河猴肾（RE2）细胞接触18 - 20小时“预处理”的伊格尔最低限度基本培养基（MEM）的恒河猴肾（RE2）细胞存在的情况下，淋病奈瑟菌的生长在接种后3 - 4小时开始，到16 - 20小时达到最大值。当从组织细胞中去除“预处理”培养基（CM）并用新鲜培养基替代时，淋病奈瑟菌开始增殖前出现5 - 6小时的延迟期，到16小时生长也接近最大值。从组织细胞中去除的CM支持6株淋病奈瑟菌的生长。3株菌在CM中不生长，但在有组织细胞存在时能增殖。没有菌株在加热杀死的组织细胞存在的情况下或仅在组织培养基中生长。在CM中生长的6株淋病奈瑟菌中，3株以其主要（90%）群体T1菌落形式存在，而其余菌株产生T1菌以及T1A, T3和T4菌落变体。这些结果表明组织细胞分泌生长因子，这些因子支持某些淋病奈瑟菌菌株T1菌落形式的生长和维持。
