Ota F, Pontefract R, Ashton F E, Diena B B
Can J Microbiol. 1975 Nov;21(11):1698-704. doi: 10.1139/m75-249.
Neisseria gonorrhoeae 188, type 1, was cultured with monolayers of Rhesus monkey kidney (RE2) cells. Attachment of gonococci to RE2 cells occurred from about 2 h after inoculation and appeared maximal by 6 h when stained with crystal violet solution and examined under a light microscope. Preferential attachment of gonococci to the perinuclear region of tissue cells could not be demonstrated. Enumeration of the colony-forming units revealed that gonococci became rapidly attached to RE2 cells, increased in number, and after 8 h gradually decreased. The number of cell-free gonococci (not bound to tissue cells) remained constant for the first few hours and then changed in parallel with the cell-bound bacterial number. Gonococci did not multiply in tissue-culture medium. When penicillin was added to the system at intervals, neither cell-free nor cell-bound gonococci were recovered. Electron microscopy of RE2 cells infected with N. gonorrhoeae revealed no intact intracellular organisms, only gonococci attached to tissue cells and imcompletely engulfed or partially digested gonococci. These observations indicate that gonococci only multiply extracellarly in the presence of RE2 cells. Furthermore, some gonococci were engulfed by RE2 cells, but appeared to be digested so rapidly that they could not multiply intracellularly.
淋病奈瑟菌188型1株与恒河猴肾（RE2）细胞单层进行培养。接种后约2小时，淋球菌开始附着于RE2细胞，6小时时用结晶紫溶液染色并在光学显微镜下观察，附着似乎达到最大值。未发现淋球菌优先附着于组织细胞的核周区域。对菌落形成单位的计数显示，淋球菌迅速附着于RE2细胞，数量增加，8小时后逐渐减少。游离淋球菌（未与组织细胞结合）的数量在最初几个小时保持恒定，然后与细胞结合的细菌数量平行变化。淋球菌在组织培养基中不繁殖。当每隔一段时间向系统中添加青霉素时，未回收游离或细胞结合的淋球菌。对感染淋病奈瑟菌的RE2细胞进行电子显微镜检查，未发现完整的细胞内生物体，仅见附着于组织细胞的淋球菌以及被不完全吞噬或部分消化的淋球菌。这些观察结果表明，淋球菌仅在RE2细胞存在的情况下在细胞外繁殖。此外，一些淋球菌被RE2细胞吞噬，但似乎被迅速消化，以至于无法在细胞内繁殖。
