• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Polypyrimidine/polypurine sequence in plasmid DNA enhances formation of dimer molecules in Escherichia coli. Dimerization of plasmid DNA in Escherichia coli.

作者信息

Kato M

机构信息

Department of Life Science, College of Integrated Arts and Sciences, University of Osaka Prefecture, Sakai, Japan.

出版信息

Mol Biol Rep. 1993 Oct;18(3):183-7. doi: 10.1007/BF01674429.

DOI:10.1007/BF01674429
PMID:8114686
Abstract

Formation of dimer molecules of a recombinant plasmid, pTIR10, which carries a pyrimidine/purine-biased stretch occurs about 6-fold more efficiently than for the control plasmid pUC19 in Escherichia coli strain JM107. Since pyrimidine/purine-biased sequences have a potential to form unusual DNA structures, this observation suggests that the inserted sequence affects the replication process of plasmid DNA, probably by forming a triple helix under physiological conditions.

摘要

相似文献

1
Polypyrimidine/polypurine sequence in plasmid DNA enhances formation of dimer molecules in Escherichia coli. Dimerization of plasmid DNA in Escherichia coli.
Mol Biol Rep. 1993 Oct;18(3):183-7. doi: 10.1007/BF01674429.
2
Structural heterogeneity of pyrimidine/purine-biased DNA sequence analyzed by atomic force microscopy.通过原子力显微镜分析嘧啶/嘌呤偏向性DNA序列的结构异质性。
Eur J Biochem. 2002 Aug;269(15):3632-6. doi: 10.1046/j.1432-1033.2002.03063.x.
3
Plasmid dimerization mediated by triplex formation between polypyrimidine-polypurine repeats.由多嘧啶-多嘌呤重复序列之间形成三链体介导的质粒二聚化。
Biochemistry. 1993 Feb 2;32(4):1072-7. doi: 10.1021/bi00055a012.
4
Sticky DNA: effect of the polypurine.polypyrimidine sequence.
J Biol Chem. 2002 Oct 18;277(42):39228-34. doi: 10.1074/jbc.M205210200. Epub 2002 Aug 2.
5
Polypurine-polypyrimidine sequences adopt unwound structure in pBR322 form V DNA as probed by single-hit analysis of HpaII sites.通过对HpaII位点的单打击分析发现，多嘌呤-多嘧啶序列在pBR322 V型DNA中呈解旋结构。
J Biomol Struct Dyn. 1993 Apr;10(5):879-90. doi: 10.1080/07391102.1993.10508681.
6
Effect of the potential triplex DNA region on the in vitro expression of bacterial beta-lactamase gene in superhelical recombinant plasmids.
J Biochem. 1992 Oct;112(4):492-4. doi: 10.1093/oxfordjournals.jbchem.a123927.
7
Structural characterization of supercoiled DNA containing a minisatellite repeat that has polypurine/polypyrimidine stretch.
Nucleic Acids Symp Ser (Oxf). 2007(51):341-2. doi: 10.1093/nass/nrm171.
8
Copy-choice illegitimate DNA recombination revisited.重访复制选择型非法DNA重组。
EMBO J. 1994 Jun 1;13(11):2725-34. doi: 10.1002/j.1460-2075.1994.tb06563.x.
9
Structure and function in Escherichia coli of plasmids containing pyrimidine/purine-biased stretch originated from the 5'-flanking region of the basidiomycete ras gene.
J Biochem. 1997 Oct;122(4):696-702. doi: 10.1093/oxfordjournals.jbchem.a021811.
10
RecQ and RecG helicases have distinct roles in maintaining the stability of polypurine.polypyrimidine sequences.RecQ解旋酶和RecG解旋酶在维持多聚嘌呤-多聚嘧啶序列的稳定性方面具有不同的作用。
Mutat Res. 2008 Aug 25;643(1-2):20-8. doi: 10.1016/j.mrfmmm.2008.05.005. Epub 2008 Jun 7.

引用本文的文献

1
Plasmid DNA vaccine vector design: impact on efficacy, safety and upstream production.质粒DNA疫苗载体设计：对效力、安全性及上游生产的影响
Biotechnol Adv. 2009 Jul-Aug;27(4):353-70. doi: 10.1016/j.biotechadv.2009.02.003. Epub 2009 Feb 20.

本文引用的文献

1
Poly(pyrimidine) . poly(purine) synthetic DNAs containing 5-methylcytosine form stable triplexes at neutral pH.含有5-甲基胞嘧啶的聚（嘧啶）·聚（嘌呤）合成DNA在中性pH下形成稳定的三链体。
Nucleic Acids Res. 1984 Aug 24;12(16):6603-14. doi: 10.1093/nar/12.16.6603.
2
Most highly repeated dispersed DNA families in the mouse genome.小鼠基因组中大多数高度重复的分散DNA家族。
Mol Cell Biol. 1984 Aug;4(8):1561-71. doi: 10.1128/mcb.4.8.1561-1571.1984.
3
The DNA sequence of the human beta-globin region is strongly biased in favor of long strings of contiguous purine or pyrimidine residues.
人类β-珠蛋白区域的DNA序列强烈偏向于连续嘌呤或嘧啶残基的长串。
Biochemistry. 1987 Dec 1;26(24):7870-5. doi: 10.1021/bi00398a050.
4
Unusual DNA structures.异常的DNA结构。
J Biol Chem. 1988 Jan 25;263(3):1095-8.
5
Practical aspects of preparing phage and plasmid DNA: growth, maintenance, and storage of bacteria and bacteriophage.
Methods Enzymol. 1987;152:145-70. doi: 10.1016/0076-6879(87)52016-x.
6
DNA H form requires a homopurine-homopyrimidine mirror repeat.DNA H型需要一个同型嘌呤-同型嘧啶镜像重复序列。
Nature. 1987;330(6147):495-7. doi: 10.1038/330495a0.
7
Topology and formation of triple-stranded H-DNA.
Science. 1989 Mar 24;243(4898):1571-6. doi: 10.1126/science.2648571.
8
Intramolecular DNA triplexes in supercoiled plasmids. I. Effect of loop size on formation and stability.超螺旋质粒中的分子内DNA三链体。I. 环大小对形成和稳定性的影响。
J Biol Chem. 1989 Apr 5;264(10):5944-9.
9
The pyrimidine/purine-biased region of the epidermal growth factor receptor gene is sensitive to S1 nuclease and may form an intramolecular triplex.表皮生长因子受体基因的嘧啶/嘌呤偏向区对S1核酸酶敏感，可能形成分子内三链体。
Biochem J. 1990 May 15;268(1):175-80. doi: 10.1042/bj2680175.
10
Triple-helical DNA pairing intermediates formed by recA protein.
J Biol Chem. 1990 Oct 5;265(28):16898-912.