Hirsch I H, Kulp-Hugues D, Sedor J, McCue P, Chancellor M B, Staas W E
Department of Urology, Jefferson Medical College, Philadelphia, PA 19107.
Paraplegia. 1993 Dec;31(12):785-92. doi: 10.1038/sc.1993.121.
Deterioration of the germinal epithelium of the testis is a known sequela of spinal cord injury (SCI) that may influence the outcome of male reproductive rehabilitation efforts. Quantitative testicular biopsy, currently regarded as the standard of assessing the integrity of spermatogenesis, has not gained wide-spread clinical use because of its invasive nature and relative technical complexity. Alternatively, aspiration DNA flow cytometry analysis of the testis has offered a potential method of spermatogenic assessment that meets both the requirements of simplicity and objectivity. The objective of this study is to determine the capability of flow cytometry to assess spermatogenesis following SCI. Eleven SCI men underwent incisional testicular biopsy with the specimen simultaneously submitted for quantitative evaluation of the germinal epithelium by both quantitative histometry and DNA flow cytometry. The haploid percentage of cells showed highly significant levels of correlation with key micrometric parameters of the quantitative testicular biopsy: spermatid/tubule (p < 0.002) and the spermatid/Sertoli cell ratio (p < 0.0005). Since tissue procurement is accomplished less invasively for flow cytometry analysis, we recommend this method as the modality of assuring integrity of the germinal epithelium in candidates for reproductive rehabilitation.
睾丸生精上皮的退化是脊髓损伤（SCI）已知的后遗症，可能会影响男性生殖康复的效果。定量睾丸活检目前被视为评估精子发生完整性的标准，但由于其侵入性和相对技术复杂性，尚未得到广泛的临床应用。相比之下，睾丸穿刺DNA流式细胞术分析提供了一种潜在的精子发生评估方法，该方法既满足简单性又满足客观性的要求。本研究的目的是确定流式细胞术评估脊髓损伤后精子发生的能力。11名脊髓损伤男性接受了切开睾丸活检，并将标本同时提交，通过定量组织学和DNA流式细胞术对生精上皮进行定量评估。细胞的单倍体百分比与定量睾丸活检的关键微观测量参数：精子细胞/曲细精管（p < 0.002）和精子细胞/支持细胞比率（p < 0.0005）显示出高度显著的相关性。由于流式细胞术分析的组织获取方式侵入性较小，我们建议将该方法作为确保生殖康复候选者生精上皮完整性的方式。
