Zwet T L, Thompson J, Furth R
Infect Immun. 1975 Oct;12(4):699-705. doi: 10.1128/iai.12.4.699-705.1975.
The effect of hydrocortisone on the phagocytosis and intracellular killing by mouse peritoneal macrophages in vitro was studied by a method making it possible to measure these processes separately. The results showed that in vivo treatment with 15 mg of hydrocortisone acetate did not significantly decrease the phagocytosis of several bacterial species such as Staphylococcus albus, Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas aeruginosa. The killing indexes of normal macrophages for the various microorganisms were found to be significantly different. This may indicate that the bactericidal mechanisms are not uniform for these bacteria. The effect of hydrocortisone on the intracellular killing was also variable. For Staphylococcus albus a normal killing index was found. For the other species of bacterial and for Candida albicans some decrease was found, but this was only significant for Salmonella typhimurium. It is concluded that a decrease host resistance due to glucocorticosterioid treatment is not caused by a direct effect of these drugs on the phagocytosis and intracellular killing by mononuclear phagocytes.
采用一种能够分别测量这些过程的方法，研究了氢化可的松对小鼠腹腔巨噬细胞体外吞噬作用和细胞内杀菌作用的影响。结果表明，用15mg醋酸氢化可的松进行体内治疗，并未显著降低几种细菌的吞噬作用，如白色葡萄球菌、金黄色葡萄球菌、大肠杆菌、鼠伤寒沙门氏菌和铜绿假单胞菌。发现正常巨噬细胞对各种微生物的杀菌指数有显著差异。这可能表明这些细菌的杀菌机制并不一致。氢化可的松对细胞内杀菌的影响也是可变的。对于白色葡萄球菌，发现其杀菌指数正常。对于其他细菌种类和白色念珠菌，杀菌指数有所下降，但仅对鼠伤寒沙门氏菌有显著意义。得出的结论是，糖皮质类固醇治疗导致宿主抵抗力下降并非这些药物对单核吞噬细胞吞噬作用和细胞内杀菌的直接作用所致。
