Celer V
Vysoká skola veterinární a farmaceutická, Brno.
Vet Med (Praha). 1993;38(11):641-6.
In the present paper antigen preparation is described to be used for diagnostics of Maedi-Visna disease by immunodiffusion test (IDT). The antigen was prepared by cultivation of the Maedi-Visna virus, OLV strain, in cell culture of the synovial membrane of carpal joint and plexus choroides of lamb. Cell cultures were used for 10-25 subcultures. The antigen was concentrated by polyethylene glycol application. The characteristics of the prepared diagnostic antigen were evaluated by its comparison with a reference, commercial preparation. Sufficient sensitivity and specificity of the prepared antigen was demonstrated by testing in a sample of 100 ovine serums.
本文描述了用于通过免疫扩散试验（IDT）诊断梅迪-维斯纳病的抗原制备方法。该抗原通过在羔羊腕关节滑膜和脉络丛的细胞培养物中培养梅迪-维斯纳病毒OLV株制备。细胞培养物传代10 - 25次。通过应用聚乙二醇对抗原进行浓缩。通过将制备的诊断抗原与参考商业制剂进行比较，评估其特性。在100份绵羊血清样本中进行检测，证明了所制备抗原具有足够的敏感性和特异性。
