Celer V, Nĕmcová H, Celer V
Department of Microbiology and Immunology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.
Folia Microbiol (Praha). 1997;42(4):395-9. doi: 10.1007/BF02816956.
The first isolation and partial characterization of ovine lentivirus in Czech Republic is described. The virus was isolated in a tissue culture system derived from plexus choroideus from sheep. The new isolate was compared with prototypic K1514 Maedi-Visna strain; these two viruses shared antigenic determinants as determined by serological testing. Both viral strains reacted in a PCR reaction with primers situated in the gag and pol gene. Based on similarities of growth characteristics, antigenic determinants and primer binding sites it can be concluded that the isolate OPM is an ovine lentivirus and is at least partly related to the prototypic Maedi-Visna strain K1514.
本文描述了在捷克共和国首次分离出绵羊慢病毒并对其进行部分特性鉴定的过程。该病毒是在源自绵羊脉络丛的组织培养系统中分离出来的。将新分离株与原型K1514梅迪 - 维斯纳毒株进行了比较；通过血清学检测确定这两种病毒具有共同的抗原决定簇。两种病毒株在PCR反应中均与位于gag和pol基因中的引物发生反应。基于生长特性、抗原决定簇和引物结合位点的相似性，可以得出结论，分离株OPM是一种绵羊慢病毒，并且至少部分与原型梅迪 - 维斯纳毒株K1514相关。
